Anti-RNF40抗體,環(huán)指蛋白40抗體二抗孵育(Secondary antibody inucubation):
參考二抗的說(shuō)明書,按照適當(dāng)比例用Western二抗稀釋液稀釋辣根過(guò)氧化物酶(HRP)標(biāo)記的二抗。
用微型臺(tái)式真空泵吸盡洗滌液,立即加入稀釋好的二抗,室溫或4℃在側(cè)擺搖床上緩慢搖動(dòng)孵育一小時(shí)?;厥斩?,加入Western洗滌液,在側(cè)擺搖床上緩慢搖動(dòng)洗滌5-10分鐘。吸盡洗滌液后,再加入洗滌液,洗滌5-10分鐘。共洗滌3次。如果結(jié)果背景較高可以適當(dāng)延長(zhǎng)洗滌時(shí)間并增加洗滌次數(shù)。
Anti-RNF40抗體,環(huán)指蛋白40抗體
英文介紹:Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction. The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s). RNF40 (RING finger protein 40), also known as BRE1B, Staring or RBP95, is a 1,001 amino acid nuclear protein that contains one RING-type zinc finger. Expressed ubiquitously with highest expression in heart, testis and pancreas, RNF40 functions as an E3 ubiquitin-protein ligase that regulates the monoubiquitination and subsequent degradation of select residues on target proteins, such as Histone H2B and Syntaxin 1. In addition, RNF40 forms a ubiquitin ligase complex with UBCH6 (an E2 enzyme) and together, these proteins play a crucial role in regulation of the histone code. Four isoforms of RNF40 exist due to alternative splicing events.
此產(chǎn)品本公司有標(biāo)記的一抗出售,相關(guān)標(biāo)記有:Alexa Fluor 350 標(biāo)記、Alexa Fluor 488 標(biāo)記、Alexa Fluor 555 標(biāo)記、Alexa Fluor 647 標(biāo)記、AP標(biāo)記、APC標(biāo)記、Biotin標(biāo)記、Cy3標(biāo)記、Cy5標(biāo)記、Cy5.5標(biāo)記、Cy7標(biāo)記、FITC標(biāo)記、Gold標(biāo)記、HRP標(biāo)記、PE標(biāo)記、PE-Cy3標(biāo)記、PE-CY5標(biāo)記、PE-CY5.5標(biāo)記、PE-CY7標(biāo)記、RBITC標(biāo)記
注意事項(xiàng):This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品名稱:Anti-RNF40抗體,環(huán)指蛋白40抗體
產(chǎn)品編號(hào):BYK-9170R
英文名稱:Anti-RNF40
中文名稱:環(huán)指蛋白40抗體
英文別名:BRE1 E3 ubiquitin ligase homolog B; BRE1-B; BRE1B; BRE1B_HUMAN; E3 ubiquitin protein ligase BRE1B; E3 ubiquitin-protein ligase BRE1B; Rb associated protein; RBP 95; RBP95; Ring finger protein 40; RNF 40; Rnf40; STARING; 95 kDa retinoblastoma associated protein; 95 kDa retinoblastoma protein binding protein; 95 kDa retinoblastoma-associated protein; BRE 1B; BRE1 B.
產(chǎn)品規(guī)格:0.1ml/0.2ml
此抗體本公司有單抗和多抗,可用于免疫組化(IHC),免疫印跡(WB),酶聯(lián)免疫(ELISA),IF、IP、流式細(xì)胞術(shù)等實(shí)驗(yàn)(可來(lái)電索取說(shuō)明)
保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
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