Anti-RNF3抗體,環(huán)指蛋白3抗體Western Blotting 具體實驗步驟：
Western，也稱Western blot、Western blotting、Western 印跡，是用抗體檢測蛋白的重要方法之一。
Western可以參考如下步驟進(jìn)行操作：
收集蛋白樣品(Protein sample preparation)
可以使用適當(dāng)?shù)牧呀庖毫呀赓N壁細(xì)胞、懸浮細(xì)胞或組織樣品。對于某些特定的亞細(xì)胞組份蛋白，例如細(xì)胞核蛋白、細(xì)胞漿蛋白、線粒體蛋白等，可以參考相關(guān)文獻(xiàn)提取這些亞細(xì)胞組份蛋白，也可以使用試劑盒進(jìn)行抽提。
收集完蛋白樣品后，為確保每個蛋白樣品的上樣量*，需要測定每個蛋白樣品的蛋白濃度。根據(jù)所使用的裂解液的不同，需要采用適當(dāng)?shù)牡鞍诐舛葴y定方法。因為不同的蛋白濃度測定方法對于一些去垢劑和還原劑等的兼容性差別很大。
產(chǎn)品名稱：Anti-RNF3抗體,環(huán)指蛋白3抗體
產(chǎn)品編號：BYK-9169R
英文名稱：Anti-RNF3
中文名稱：環(huán)指蛋白3抗體
英文別名：DONG1; PCGF3; polycomb group ring finger 3; Ring finger protein 3; RNF3; RNF3A; PCGF3_HUMAN.
產(chǎn)品規(guī)格：0.1ml/0.2ml
此抗體本公司有單抗和多抗，可用于免疫組化（IHC），免疫印跡（WB），酶聯(lián)免疫(ELISA)，IF、IP、流式細(xì)胞術(shù)等實驗（可來電索取說明）
保存條件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Anti-RNF3抗體,環(huán)指蛋白3抗體
英文介紹：Polycomb group (PcG) proteins form multiprotein complexes that regulate expression patterns of developmental and cell proliferation genes. Several members of the PcG contain ring finger domains and are identified as a subclass of RING finger proteins. The RING-type zinc finger motif is present in a number of viral and eukaryotic proteins and is made of a conserved cysteine-rich domain that is able to bind two zinc atoms. Proteins that contain the RING-type zinc finger conserved domain are generally involved in the ubiquitination pathway of protein degradation. PCGF3 (polycomb group ring finger 3), also known as RNF3, DONG1 or RNF3A, is a 242 amino acid transcriptional regulator that is encoded by a gene located on human chromosome 4, which encodes nearly 6% of the human genome and has the largest gene deserts (regions of the genome with no protein encoding genes) of all of the human chromosomes. PCGF3 exists as two isoforms produced by alternative splicing events.
此產(chǎn)品本公司有標(biāo)記的一抗出售，相關(guān)標(biāo)記有：Alexa Fluor 350 標(biāo)記、Alexa Fluor 488 標(biāo)記、Alexa Fluor 555 標(biāo)記、Alexa Fluor 647 標(biāo)記、AP標(biāo)記、APC標(biāo)記、Biotin標(biāo)記、Cy3標(biāo)記、Cy5標(biāo)記、Cy5.5標(biāo)記、Cy7標(biāo)記、FITC標(biāo)記、Gold標(biāo)記、HRP標(biāo)記、PE標(biāo)記、PE-Cy3標(biāo)記、PE-CY5標(biāo)記、PE-CY5.5標(biāo)記、PE-CY7標(biāo)記、RBITC標(biāo)記
注意事項:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Anti-RNF3抗體,環(huán)指蛋白3抗體產(chǎn)品現(xiàn)貨銷售，其他同類產(chǎn)品：
byk-10459R γ-谷氨酰轉(zhuǎn)肽酶7輕鏈抗體 Anti-GGT7 light chain
byk-0459R 3-磷酸甘油醛脫氫酶（兔來源免疫組化用抗體） Anti-GAPDH
byk-1641R 磷酸化神經(jīng)生長相關(guān)蛋白43抗體 Anti-phospho-GAP43(Ser41)
byk-11641R 胎兒阿茲海默病抗原/核小體重塑因子抗體 Anti-BPTF/FALZ
byk-0154R 神經(jīng)生長相關(guān)蛋白43 Anti-GAP43
byk-10154R 磷酸化細(xì)胞轉(zhuǎn)錄因子ELK1抗體 Anti-Phospho-ELk1(Ser383)
byk-11699R 神經(jīng)性舞蹈病蛋白抗體 Anti-Huntingtin
byk-1699R 半乳糖凝集素9抗體 Anti-Galectin 9
byk-0721R 半乳糖凝集素3抗體 Anti-Galectin 3
byk-0017M 神經(jīng)節(jié)肽抗體 Anti-Galanin
byk-11361R 突觸相關(guān)蛋白97抗體 Anti-SAP97
byk-1361R GADD153抗體 Anti-GADD153/CHOP/DDIT3
byk-11360R 神經(jīng)元突觸膜胞外分泌調(diào)節(jié)蛋白4抗體 Anti-RIMS4
byk-1360R 生長抑制DNA損傷基因45抗體 Anti-GADD45
byk-11776R 人狀瘤病毒E6相關(guān)蛋白抗體 Anti-UBE3A/E6-AP
byk-1776R 生長抑制DNA損傷基因34抗體 Anti-GADD34
byk-10400R 蓬亂蛋白2抗體 Anti-shevelled 2
byk-0400R *脫羧酶-65抗體(C端） Anti-GAD65
byk-11302R NK2轉(zhuǎn)錄樣蛋白B抗體 Anti-Nkx2.2/NKX2-2
byk-1302R *脫羧酶67抗體 Anti-GAD67/GAD1
byk-10325R 磷酸化組蛋白去乙酰化酶4(Ser246)抗體 Anti-Phospho-HDAC4 (Ser246)
byk-0325R *脫羧酶-65抗體 Anti-GAD65
byk-11293R 同源盒基因HOXA4蛋白抗體 Anti-HOXA4
byk-1293R G氨基丁酸B型受體2抗體 Anti-GABBR2/GABA B Receptor 2
byk-0533R gamma氨基丁酸B型受體1抗體 Anti-GABABR1/GABA B Receptor 1
byk-11232R RecQ解旋酶樣蛋白5抗體 Anti-RECQL5
byk-1232R G1氨基丁酸A型受體α1抗體 Anti-GABRA1/GABA A Receptor alpha 1
byk-0348R G蛋白/鳥苷酸結(jié)合蛋白抗體 Anti-G protein beta subunit GI
咨詢及訂購的方法：
1.可來電向我們直接咨詢及訂購。：021-6034 0820,158 0061 4926
2.可加客服或交談，向我司在線人員咨詢及訂購。
3.可通過郵件咨詢及訂購。
環(huán)保在線