Anti-CD28抗體,CD28抗體抗體溶解方法及分裝：

1、購買抗體后，查看標簽上的規(guī)格，如：0.1ml、0.2ml
2、以規(guī)格為 0.1ml 的凍干粉抗體為例（部分抗體為液體，無需再溶解）：
溶解方法一：用移液器加 0.1ml 雙蒸水到試管內(nèi)，混勻，-20℃保存，若為其它規(guī)格（如：0.2ml）的凍干粉抗體，則加與標簽上規(guī)格*的（如：0.2ml）雙蒸水溶解，
混勻，-20℃保存。
溶解方法二：用移液器加 0.05ml（規(guī)格的 50%）雙蒸水到試管內(nèi)，再加 0.05ml（規(guī)格的 50%）
的甘油到試管內(nèi)，混勻，-20℃保存，若為其它規(guī)格（如：0.2ml）的凍干粉抗體，則加標簽上規(guī)格的 50%（如：0.1ml）雙蒸水，再加
標簽上規(guī)格的 50%（如：0.1ml）甘油溶解，混勻，-20℃保存。
抗體溶解后，將其分裝成 5-10 支（以 0.1ml 抗體為例，20ulx5 支或 10ulx10 支）， -20℃保存，用
一支取一支，避免反復凍溶。
另：預試驗可做幾個梯度 1：100、1：200、1：400 稀釋，如果背景高，還可再稀釋，選一個zui
佳的稀釋比例，再正式做，效果。

產(chǎn)品名稱:Anti-CD28抗體,CD28抗體

產(chǎn)品編號：BYK-8865R
英文名稱：Anti-CD28
中文名稱：CD28抗體
英文別名：CD 28; CD28 antigen; CD28 molecule; MGC138290; T cell antigen CD28; T cell specific surface glycoprotein; T cell specific surface glycoprotein CD28; Tp44; CD28_HUMAN.
產(chǎn)品規(guī)格：0.1ml/0.2ml
此抗體本公司有單抗和多抗，可用于免疫組化（IHC），免疫印跡（WB），酶聯(lián)免疫(ELISA)，IF、IP、流式細胞術(shù)等實驗（可來電索取說明）
保存條件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Anti-CD28抗體,CD28抗體

英文介紹：Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. SUBUNIT: Homodimer; disulfide-linked. Interacts with DUSP14. Binds to CD80/B7-1 and CD86/B7-2/B70. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY: Expressed in T-cells and plasma cells, but not in less mature B-cells. Human CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. It is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes (approximay 95% of CD4+ cells and 50% of CD8+ cells). Mature thymocytes exhibit higher levels of CD28 than the immature cells. Activation of T cells results in enhanced CD28 expression. T cell activation requires two combined signals provided by antigen presenting cells. The first is mediated via the T cell receptor following its interaction with antigenic peptide MHC complexes, and the second is delivered by accessory or costimulating molecules through their counter receptors on T lymphocytes. CD28 bears structural homology to CTLA 4 which is expressed at very low levels on the surface of CD4+ and CD8+ peripheral blood cells only following activation. CD28 is the natural receptor for the B7/BB 1 ligand (CD80) a 55-60 kDa glycoprotein which is expressed on activated B lymphocytes, on dendritic cells and on interferon gamma treated monocytes. The binding of B7 1/BB 1 molecules to CD28 is involved in T lymphocyte activation and in the initiation and maintenance of chronic inflammation.
此產(chǎn)品本公司有標記的一抗出售，相關(guān)標記有：Alexa Fluor 350 標記、Alexa Fluor 488 標記、Alexa Fluor 555 標記、Alexa Fluor 647 標記、AP標記、APC標記、Biotin標記、Cy3標記、Cy5標記、Cy5.5標記、Cy7標記、FITC標記、Gold標記、HRP標記、PE標記、PE-Cy3標記、PE-CY5標記、PE-CY5.5標記、PE-CY7標記、RBITC標記
注意事項:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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