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BACKGROUND
DNA or RNA damage can hinder the ability of a cell to carry out its function and can significantly increase the likelihood of tumor formation. One of the causes of damaged DNA and RNA is oxidation of the bases. 8hydroxy2'deoxyguanosine, 8hydroxyguanine （8OHdG） and 8hydroxyguanosine are all markers of oxidative damage to RNA and DNA. 8hydroxy2'deoxyguanosine is produced by reactive oxygen and nitrogen species, including hydroxyl radical and peroxynitrite. 8hydroxyguanine is one of the major base lesions involved in mutagenesis and is caused by ionizing radiation and radiomimetic agents. 8hydroxyguanosine induces a transversion of G to T in DNA, which may be mutagenic. Markers of DNA and RNA damage are useful research tools when studying the effects of this type of damage.
REFERENCES
1. Musarrat, J., et al. 1996. Prognostic and aetiological relevance of 8hydroxyguanosine in human breast carcinogenesis. Eur. J. Cancer 32: 12091214
2. Parker, A.R., et al. 2002. 8hydroxyguanosine repair is defective in some microsalite stable colorectal cancer cells. Cancer Res. 62: 72307233.
3. Abe, T., et al. 2002. Alteration of 8hydroxyguanosine concentrations in the cerebrospinal fluid and serum from patients with Parkinson’s disease. Neurosci. Lett. 336: 105108.
4. Winter, D.B., et al. 2003. Normal somatic hypermutation of Ig genes in the absence of 8hydroxyguanineDNA glycosylase. J. Immunol. 170: 55585562.
5. Russo, M.T., et al. 2004. Accumulation of the oxidative base lesion 8hydroxyguanine in DNA of tumorprone mice defective in both the MYH and OGG1 DNA glycosylases. Cancer Res. 64: 44114414.
6. Okugawa, Y., et al. 2006. UVAinduced degradation of 8hydroxyguanine in oligonucleotide and the effect on its activities in yeast oligonucleotide transformation assay. Nucleic Acids Symp. Ser. 48: 287288.
7. Watanabe, E., et al. 2006. Significance of 8hydroxy2'deoxyguanosine levels in patients with idiopathic dilated cardiomyopathy. J. Card. Fail. 12: 527532.
8. Noblitt, S.D., et al. 2007. The role of metal ion binding in generating 8hydroxy2'deoxyguanosine from the nucleoside 2'deoxyguanosine and the nucleotide 2'deoxyguanosine5'monophosphate. J. Inorg. Biochem.
101: 536542.
9. Kuo, H.W., et al. 2007. Urinary 8hydroxy2'deoxyguanosine （8OHdG） and genetic polymorphisms in breast cancer patients. Mutat. Res. 631: 6268.
APPLICATIONS
8OHdG （J1） is recommended for detection of 8OHdG modified proteins by Western Blotting （starting dilution 1:200, dilution range 1:1001:1000）, immunofluorescence （starting dilution 1:50, dilution range 1:501:500） and solid phase ELISA （starting dilution 1:30, dilution range 1:301:3000）.
RESEARCH USE
For research use only, not for use in diagnostic procedures.
RECOMMENDED SECONDARY REAGENTS
To ensure optimal results, the following support （secondary） reagents are recommended: 1） Western Blotting: use goat antirabbit IgGHRP: sc2004 （dilution range: 1:20001:100,000） or Cruz Marker™ compatible goat antirabbit IgGHRP: sc2030 （dilution range: 1:20001:5000）, Cruz Marker™ Molecular Weight Standards: sc2035, TBS Blotto A Blocking Reagent: sc2333 and Western Blotting Luminol Reagent: sc2048. 2） Immunofluorescence: use goat antirabbit IgGFITC: sc2012 （dilution range: 1:1001:400） or goat antirabbit IgGTR: sc2780 （dilution range: 1:1001:400） with UltraCruz™ Mounting Medium: sc24941.
