*化01群小川型霍亂弧菌抗體IgG

【資料簡介】

*化01群小川型霍亂弧菌抗體IgG

Anti-V.cholera Ogawa/Biotin  *化01群小川型霍亂弧菌抗體IgG

Anti-Influenza A Virus H1/HRP  辣根過氧化物酶標記羊抗季節性A型流感病毒H1抗體IgG

Anti-Influenza A Virus H3/HRP  辣根過氧化物酶標記羊抗季節性流感病毒H3抗體IgG

Anti-H1N1-A/HRP（Influenza A virus）California  辣根過氧化物酶標記兔抗季節性A型人流感病毒H1N1抗體IgG（美國加州型）

Anti-CD4/Biotin  *化CD4抗體IgG

Anti-VDAC/FITC  熒光素標記電壓依賴性陰離子通道抗體IgG

Anti-CD14/Biotin  *化CD14抗體IgG

Anti-VEGF/FITC  熒光素標記VEGF抗體IgG

Anti-VEGF（Rabbit）/FITC  熒光素標記血管內皮生長因子抗體IgG

Anti-VEGF-A/FITC  熒光素標記血管內皮生長因子A抗體IgG

Anti-VEGF-B/FITC  熒光素標記血管內皮生長因子B抗體IgG

Anti-VEGF-C/FITC  熒光素標記血管內皮生長因子C型抗體IgG

Anti-VEGF/RBITC  羅丹明標記VEGF抗體IgG

Mouse Anti-human VEGF/FITC  熒光素標記小鼠抗人VEGF單克隆抗體IgG

Anti-VEGFR1/VEGF-R1/Flt-1 /FITC  熒光素標記血管內皮生長因子受體1抗體IgG

Anti-VEGFR2（VEGF-R2）Flk1/FITC  熒光素標記血管內皮生長因子受體2抗體IgG

Anti-SV2A/FITC  熒光素標記突觸泡蛋白2A抗體IgG

Assemble "sandwich" for Bio-Rad's Transblot. 
Prewet the sponges, filter papers （slightly bigger than gel） in 1x Blotting buffer.
Sponge - filter paper - gel - membrane - filter paper - sponge 
Transfer for 1 hr at 1 amp at 4°C on a stir plate. 
Bigger proteins might take longer to transfer. 
For the Mini-Transblot, it's 100 V for 1 hr with the cold pack and prechilled buffer. 
When finished, immerse membrane in Blocking buffer and block overnight. 

H. Antibodies and detection 

Incubate with primary antibody diluted in Blocking buffer for 60 min at room temp. 
Wash 3 x 10 min with 0.05% Tween 20 in PBS. 
Incubate with secondary antibody diluted in Blocking buffer for 45 min at room temp. 
Wash 3 x 10 min with 0.05% Tween 20 in PBS. 
Detect with Amersham ECL kit （RPN 2106）. 

I. Stripping blot 

Rinse blot off with 0.05% Tween 20 in PBS. 
Put blot into Kapak bag cut to slightly bigger size than blot. 
Add about 5 to 10 ml Stripping buffer. 
Remove as much air as possible and seal bag. 
Immerse into 80°C water bath and incubate for 20 min. 
Rinse blot off with 0.05% Tween 20 in PBS. 
Block for about 1 hr with 5% BSA/Tween 20, or overnight with 3% BSA/Tween 20.