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閱讀：250發(fā)布時(shí)間：2013-4-14
Rabbit Anti-CD25抗體說(shuō)明書(shū)
Cat. Number: Rs-0577R
Quantity size: 0.2ml （dilute with pH 7.4 0.01 M PBS or diluent of antibody）
Background: CD25（IL2） receptor alpha （IL2RA） and beta （IL2RB） chains, together with the common gamma chain （IL2RG）, constitute the high-affinity IL2 receptor. Homodimeric alpha chains（IL2RA） result in low-affinity receptor, while homodimeric beta （IL2RB） chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternay-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown.
Specificity: ·anti- CD25 is a rabbit monoclonal antibody unconjugated
·specific for CD25 of rat
·epitope mapping near the amino terminus of CD25 of
rat origin
· use for western blotting, elisa, immunoprecipitation
and immunohistochemistry
· Protein A affinity chromatography purification, purity :>95%
· Isotype: IgG
· mol wt: 82kDa
Application: · Western blotting: 1:100-500
· Immunohistochemistry: 1:100-500
· ELISA: 1:500-1000
· Optimal working dilutions must be determined by the end user.
Storage: Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ºC.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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