ActiveGαoGTP[26907][30µL]CatalogNumber:26907GeneSymbol:GnaoDescription:AntiActiveGαoMouseMonoclonalAntibodyBackground:HeterotrimericGproteinsarecriticalcellularsignaltransducers

詳細介紹

Active Gαo GTP [26907]
[30 µL]

Catalog Number: 26907

Gene Symbol: Gnao

Description: Anti Active Gα_o Mouse Monoclonal Antibody

Background: Heterotrimeric G proteins are critical cellular signal transducers. Gα_orepresents one sub-family of G proteins that could mediate the inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gα_o proteins are being explored.

Immunogen: Recombinant full length protein of active Gα_o

Applications: IP, IHC

Recommended Dilutions: 1 µg for 1~2 mg total cellular proteins for IP;
1:100 dilution for IHC

Concentration: 1 mg/ml

Host: mouse

Format: Liquid

Clonality: Monoclonal

Isotype: IgG1

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg²⁺ ａnd Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Anti-active Gα_o antibody recognizes active Gα_o of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Download Datasheet:

Immunoprecipitation/Western blot:

Gα_o activation assay. Purified Gα_o proteins were loaded as a control (lanes 1) or immunoprecipitated after treated with GDP (lane 2) or GTPγS (lane 3). Immunoprecipitation was done with the anti active Gα_o monoclonal antibody (Cat. No. 26907). Immunoblot was with an anti Gα_opolyclonal antibody (Cat. No. 21015).

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