Vimentin[26054][100µL]CatalogNumber:26054GeneSymbol:VIMDescription:AntiVimentinMouseMonoclonalAntibodyBackground:Vimentinisanintermediatefilamentproteinfoundinvariousnon-epithelialcells

詳細介紹

Vimentin [26054]
[100 µL]

Catalog Number: 26054

Gene Symbol: VIM

Description: Anti Vimentin Mouse Monoclonal Antibody

Background: Vimentin is an intermediate filament protein found in various non-epithelial cells, especially in mesenchymal cells. It is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both normal development and metastatic progression.

Immunogen: A synthetic peptide from the internal region of human vimentin protein.

Applications: ELISA, WB, IHC, IP

Recommended Dilutions:
ELISA: 1:1000-1:5000
WB: 1:100-1:1000
IHC: 1:50-1:100

Concentration: 1 mg/ml

Host: mouse

Format: Liquid

Clonality: Monoclonal

Isotype: IgG

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg²⁺ ａnd Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Anti vimentin antibody recognizes vimentin of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Download Datasheet:

Western blot:

Western blot analysis of recombinant vimentin protein. Purified His-tagged vimentin protein (Cat.#10190) was blotted with anti vimentin monoclonal antibody (Cat.# 26054).

Immunohistochemical analysis of paraffin-embedded human renal cancer tissue

with anti-vimentin monoclonal antibody (Cat. #26054). Tissue samples were fixed

with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:100 dilution) overnight at 4°C.A HRP-conjugated Goat anti-mouse IgG (1:50 dilution) was used as secondary antibody

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