CTNNB1(G34V)[26299][100ul]CatalogNumber:26299GeneSymbol:Beta-catenin,CTNNB,Cateninbeta-1Description:AntiCTNNB1(G34V)MouseMonoclonalAntibodyBackground:CTNNB1proteinisadualfunctionprotein

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CTNNB1(G34V) [26299]
[100ul]

Catalog Number: 26299

Gene Symbol: Beta-catenin, CTNNB, Catenin beta-1

Description: Anti CTNNB1(G34V) Mouse Monoclonal Antibody

Background: CTNNB1 protein is a dual function protein. It is a subunit of a complex of proteins that from adherens junctions, which are important for the establishment and maintenance of epithelial cell layers by regulating cell growth and adhesion between adjacent cells. CTNNB1 protein also pulls double duty as an intracellular signal transducer in the Wnt signaling pathway. Mutant CTNNB1(?-catenin) has been implicated in the pathogenesis of several cancers including melanoma, colorectal cancer, hepatocelluar carcinoma, and ovarian cancerMutations of CTNNB1 have been implicated in the pathogenesis of several cancers.

Immunogen: A synthetic peptide from the internal region of CTNNB1 which includes the mutation of G34V, human origin.

Applications: ELISA, WB, IHC

Recommended Dilutions:
ELISA: 1:1000-1:2000
WB: 1:500-1:1000
IHC: 1:50-1:100

Concentration: 0.5 mg/ml

Host: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype:

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes G34V mutant, but not wild-type CTNNB1 of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Download Datasheet:

Western blot:

Western blot analysis of recombinant CTNNB1(G34V) and wildtype proteins. Purified His-tagged CTNNB1(G34V) protein (lane2) and corresponding wild-type protein (lane1) were blotted with Anti CTNNB1(G34V) monoclonal antibody (Cat. #26299).

Immunofluorescence:

Immunofluorescence of cells expressing CTNNB1 proteins with Anti CTNNB1(G34V) antibody. HEK293T cells were transfected with pCDNA3-GFP-CTNNB1(G34V) plasmid, pCDNA3-GFP-CTNNB1(WT) plasmid or pCDNA3-GFP-CTNNB1(G34E) plasmid, then fixed and stained with Anti CTNNB1(G34V) monoclonal antibody (Cat. #26299).

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