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武漢紐斯特生物科技有限公司
武漢紐斯特生物科技有限公司
Gαi[26003][100µL]CatalogNumber:26003GeneSymbol:GnaiDescription:AntiGαiMouseMonoclonalAntibodyBackground:HeterotrimericGproteinsarecriticalcellularsignaltransducers
Gαi [26003]
[100 µL]
| Catalog Number: 26003 |
| Gene Symbol: Gnai |
| Description: Anti Gαi Mouse Monoclonal Antibody |
| Background: Heterotrimeric G proteins are critical cellular signal transducers. Gai represents one sub-family of G proteins that could mediate the inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gai proteins are being explored. |
| Immunogen: Recombinant full length protein of Gαi1 |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA 1:1000-1:5000,WB 1:1000-1:2000,IHC 1:100-1:200 |
| Concentration: 1 mg/ml |
| Host: mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ ａnd Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Gαi antibody recognizes Gαi1, Gαi2, and Gαi3 of vertebrates |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Download Datasheet: |
| Western blot: |
| Western blot analysis of Gi proteins. Gαi1 plasmid DNA was transiently transfected into HEK-293 cells. Whole-cell lysate was used for western blots. The monoclonal anti-Gαi antibody was pre-incubated without (lane 1) or with (lane 2) the antigen protein (Gαi1) to show the specificity. |
| Related Products | |||
| Catalog# | Name | Size | Price |
| Gαi Activation Assay Kit | 20 assays | ￥6800 | |
| Active Gαi-GTP Monoclonal Antibody | 30 μL | ￥4800 | |
| Anti-Gαi Mouse Monoclonal Antibody | 100 μL | ￥2800 | |
| Anti-Gαi Rabbit Polyclonal Antibody | 100 μL | ￥2600 | |
| Publications: |
| 1. Biochem J. 2013 Feb 15;450(1):209-19 |
| 2. J Clin Endocrinol Metab. 2012 Oct;97(10):E1851-61 |
| 3. Cancer Res. 2012 Jan 1;72(1):122-32 |
| 4. Endocrinology. 2012 Oct;153(10):4918-28 |
| 5. Cell Signal. 2011 Mar;23(3):550-4 |
| 6. Biochim Biophys Acta. 2011 Mar;1813(3):466-72 |
| 7. Steroids Volume 77, Issue 5, April 2012, Pages 424–432 |
| 8. J Biol Chem. 2010 Aug 27;285(35):26976-86 |
| 9. Proc Natl Acad Sci U S A. 2010 Jan 12;107(2):628-32 |
| 10. Mol Biol Cell. 2014 Nov 5;25(22):3654-71 |
| 11. Mol Cell Biol. 2014 Nov 15;34(22):4186-99 |
| 12. J Biol Chem. 2014 May 2;289(18):12748-58 |
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