污水處理設(shè)備 污泥處理設(shè)備 水處理過濾器 軟化水設(shè)備/除鹽設(shè)備 純凈水設(shè)備 消毒設(shè)備|加藥設(shè)備 供水/儲水/集水/排水/輔助 水處理膜 過濾器濾芯 水處理濾料 水處理劑 水處理填料 其它水處理設(shè)備
武漢紐斯特生物科技有限公司
會員1.png)
武漢紐斯特生物科技有限公司
產(chǎn)品型號
品 牌
廠商性質(zhì)生產(chǎn)商
所 在 地
聯(lián)系方式：樂亮明查看聯(lián)系方式
更新時間：2024-04-27 09:36:07瀏覽次數(shù)：43次
聯(lián)系我時，請告知來自 環(huán)保在線ActiveRac1-GTP[26903][30µL]CatalogNumber:26903GeneSymbol:RAC1Description:AntiActiveRac1GTPMouseMonoclonalAntibodyBackground:SmallGTPasesareasuper-familyofcellularsignalingregulators
Active Rac1-GTP [26903]
[30 µL]
Catalog Number: 26903
| Gene Symbol: RAC1 |
| Description: Anti Active Rac1 GTP Mouse Monoclonal Antibody |
| Background: Small GTPases are a super-family of cellular signaling regulators. Rac belongs to the Rho sub-family of GTPases that regulate cell motility, cell division, and gene transcription. GTP binding increases the activity of Rac, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs). |
| Immunogen: Recombinant full length protein of active Rac1 |
| Applications: IP, IHC |
| Recommended Dilutions: 1 µg for 1~2 mg total cellular proteins for IP; 1:100 dilution for IHC |
| Concentration: 1 mg/ml |
| Host: mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgM |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ ａnd Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Anti active Rac1 antibody recognizes active Rac1 from vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Download Datasheet: |
| Immunoprecipitation/Western blot: |
| Rac activation assay. MEF cells were treated with (lane 2) or without (lane 1) PDGF. Cell lysates were incubated with an anti active Rac monoclonal antibody (Cat # 26903) (top panel). Th precipitated active Rac was immunoblotted with an anti Rac rabbit polyclonal antibody (Cat # 21003). The bottom panel shows the Western blot with anti Rac of the cell lysates used (5% of that used in the top panel). |
| Immunofluorescence: |
| Immunofluorescence for the active Rac using Anti Active Rac1 GTP Mouse Monoclonal Antibody [26903] shows Rac GTP immunolabeling (green) in combination with cofilin (red) on brain tissue sections. The tissue sections were fixed with -20 oC methanol or 4% paraformaldehyde (fixation time 1hr) and stained with antibody at 1:1000 in 0.1M Phosphate buffer with 0.3% Triton X, and 4% BSA for 24h at room temperature. Secondary antibodies were anti-mouse AlexaFluor488 and anti-rabbit AlexaFluor594 at 1:1000. |
Related Products
| Catalog# | Name | Size | Price |
| Rac Activation Assay Kit | 20 assays | ￥6800.00 | |
| Active Rac1-GTP Monoclonal Antibody | 30 μL | ￥4800.00 | |
| Anti-Rac1 Mouse Monoclonal Antibody | 100 μL | ￥2800.00 | |
| Anti-Rac1 Rabbit Polyclonal Antibody | 100 μL | ￥2600.00 | |
| Anti-Rac2 Rabbit Polyclonal Antibody | 100 μL | ￥2600.00 |
| Publications: |
| 1. Cytoskeleton Volume 71, Issue 4, pages 257–272, April 2014 |
| 2. Neutrophil Methods and Protocols Methods in Molecular Biology Volume 1124, 2014, pp 251-268 |
| 3. Molecular and Cellular Neuroscience Volume 60, May 2014, Pages 10–25 |
| 4. The Journal of Immunology April 14, 2014 1301155 |
| 5. International Journal of Cancer Volume 135, Issue 11, pages 2493–2506, 1 December 2014 |
| 6. J Biol Chem. 2014 May 30;289(22):15244-58 |
| 7. Mol Cell Biol. 2014 Nov 15;34(22):4186-99 |
| 8. PLoS One. 2014 Sep 23;9(9):e108257 |
| 9. Cancer Lett. 2015 Jan 28;356(2 Pt B):382-91 |
| 10. Physiological ReportsPublished 24 October 2014Vol. 2no. e12175DOI: 10.14814/phy2.12175 |
| 11. Anticancer Research December 2014 vol. 34 no. 12 7111-7117 |
| 12. Cancer letters. March 1, 2015Volume 358, Issue 1, Pages 76–84. |
| 13. Integr Biol (Camb). 2014 Dec 24 |
| 14. Mol Neurobiol. 2015 Jan 24 |
您感興趣的產(chǎn)品PRODUCTS YOU ARE INTERESTED IN
小鼠信號轉(zhuǎn)導(dǎo)分子7（Smad7）ELISA試劑盒
KJ-2748 面議
犬內(nèi)皮型一氧化氮合成酶（eNOS）ELISA試劑盒
48T/96T 面議環(huán)保在線 設(shè)計制作，未經(jīng)允許翻錄必究 .? ? ?
請輸入賬號
請輸入密碼
請輸驗(yàn)證碼
