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Anti MEK1(F53L) Mouse Monoclonal Antibody

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MEK1(F53L)[26263][100ul]CatalogNumber:26263GeneSymbol:MAP2K1

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MEK1(F53L) [26263]
[100ul]

Catalog Number: 26263

Gene Symbol: MAP2K1; MAPKK1; MEK1; MKK1; PRKMK1

Description: Anti MEK1(F53L) Mouse Monoclonal Antibody

Background: MEK1 (also known as extracellular signal-regulated kinases, ERKs) plays important role in the MAP kinase cascade, which transduces multiple extracellular signals to control cell growth, proliferation and differentiation. Activated upon growth factors stimulation, MEK1 phosphorylates MAPK3/ERK1 and MAPK1/ERK2, thus activates the MAP pathway and regulates transcription. Abnormal of the MEK1 protein, including point mutations, are implicated in diseases such as cardiofaciocutaneous syndrome (CFC) and melanoma.

Immunogen: A synthetic peptide from the internal region of MEK1 which includes the mutation of F53L, human origin.

Applications: ELISA, WB, IHC

Recommended Dilutions:
ELISA: 1:1000-1:2000
WB: 1:500-1:1000
IHC: 1:50-1:100

Concentration: 1 mg/ml

Host: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype:

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg²⁺ ａnd Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes F53L mutant, but not wild-type MEK1 of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Download Datasheet:

Western blot:

Western blot analysis of recombinant MEK1(F53L) and wildtype proteins. Purified His-tagged MEK1(F53L) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with Anti MEK1(F53L) monoclonal antibody (Cat. #26263).

Immunofluorescence:

Immunofluorescence of cells expressing MEK1 proteins with Anti MEK1(F53L) antibody. HEK293T cells were transfected with pCDNA3-GFP MEK1(WT) plasmid (left column) or pCDNA3-GFP-MEK1(F53L) plasmid (right column), then fixed and stained with Anti MEK1(F53L) monoclonal antibody (Cat. #26263).

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