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武漢紐斯特生物科技有限公司
FBXW7(R479L)[26347][100ul]CatalogNumber:26347GeneSymbol:FBW6
FBXW7(R479L) [26347]
[100ul]
Catalog Number: 26347 |
Gene Symbol: FBW6; FBW7; FBXW7; FBX30; CDC4; SEL10 |
Description: Anti FBXW7(R479L) Mouse Monoclonal Antibody |
Background: F-box/WD repeat-containing protein 7 is a protein that in humans is encoded by the FBXW7 gene. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. Mutations in this gene are detected in ovarian, breast and colorectal cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers. |
Immunogen: A synthetic peptide from the internal region of FBXW7 which includes the mutation of R479L, human origin. |
Applications: ELISA, WB |
Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:1000 |
Concentration: 1 mg/ml |
Host: Mouse |
Format: Liquid |
Clonality: Monoclonal |
Isotype: |
Purity: Purified from ascites |
Storage buffer: |
Preservative: no |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
Species Reactivity: Recognizes R479L mutant, but not wild-type FBXW7 of vertebrates. |
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
Download Datasheet: |
Western blot: |
Western blot analysis of recombinant FBXW7(R479L) and wildtype proteins. Purified His-tagged FBXW7(R479L) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with Anti FBXW7(R479L) monoclonal antibody (Cat. #26347). |
Immunofluorescence: |
Immunofluorescence of cells expressing FBXW7 proteins with Anti FBXW7(R479L) antibody. HEK293T cells were transfected with pCDNA3-GFP FBXW7(WT) plasmid (left column) or pCDNA3-GFP FBXW7(R479L) plasmid (right column), then fixed and stained with Anti FBXW7(R479L) monoclonal antibody (Cat. #26347). |
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