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武漢紐斯特生物科技有限公司
武漢紐斯特生物科技有限公司
KIT(W557R)[26275][100ul]CatalogNumber:26275GeneSymbol:C-Kit
KIT(W557R) [26275]
[100ul]
| Catalog Number: 26275 |
| Gene Symbol: C-Kit; CD117; KIT; PBT; SCFR |
| Description: Anti KIT(W557R) Mouse Monoclonal Antibody |
| Background: Tyrosine-protein kinase Kit, also known as proto-oncogene c-Kit or CD117 or Mast/stem cell growth factor receptor (SCFR), is a receptor tyrosine kinase protein that in humans is encoded by the KIT gene. Activating mutations in this gene are associated with gastrointestinal stromal tumors, testicular seminoma, mast cell disease, melanoma, acute myeloid leukemia, while inactivating mutations are associated with the genetic defect piebaldism. |
| Immunogen: A synthetic peptide from the internal region of KIT which includes the mutation of W557R, human origin. |
| Applications: ELISA, WB, IF, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IF: 1:50-1:100 IHC: 1:25-1:100 |
| Concentration: 1 mg/ml |
| Host: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes W557R mutant, but not wild-type KIT of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Download Datasheet: |
| Western blot: |
| Western blot analysis of recombinant KIT(W557R) and wildtype proteins. Purified His-tagged KIT(W557R) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with Anti KIT(W557R) monoclonal antibody (Cat. #26275). |
| Immunofluorescence: |
| Immunofluorescence of cells expressing KIT proteins with Anti KIT(W557R) antibody. HEK293T cells were transfected with pCDNA3-GFP KIT(WT) plasmid (left column) or pCDNA3-GFP KIT(W557R) plasmid (right column), then fixed and stained with Anti KIT(W557R) monoclonal antibody (Cat. #26275). |
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