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武漢紐斯特生物科技有限公司
武漢紐斯特生物科技有限公司
MEK1(P264S)[26266][100ul]CatalogNumber:26266GeneSymbol:MAP2K1
MEK1(P264S) [26266]
[100ul]
| Catalog Number: 26266 |
| Gene Symbol: MAP2K1; MAPKK1; MEK1; MKK1; PRKMK1 |
| Description: Anti MEK1(P264S) Mouse Monoclonal Antibody |
| Background: MEK1 (also known as extracellular signal-regulated kinases, ERKs) plays important role in the MAP kinase cascade, which transduces multiple extracellular signals to control cell growth, proliferation and differentiation. Activated upon growth factors stimulation, MEK1 phosphorylates MAPK3/ERK1 and MAPK1/ERK2, thus activates the MAP pathway and regulates transcription. Abnormal of the MEK1 protein, including point mutations, are implicated in diseases such as cardiofaciocutaneous syndrome (CFC) and melanoma. |
| Immunogen: A synthetic peptide from the internal region of MEK1 which includes the mutation of P264S, human origin. |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:2000 WB: 1:500-1:1000 IHC: 1:50-1:100 |
| Concentration: 0.3 mg/ml |
| Host: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ ａnd Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes P264S mutant, but not wild-type MEK1 of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Download Datasheet: |
| Western blot: |
| Western blot analysis of recombinant MEK1(P264S) and wildtype proteins. Purified His-tagged MEK1(P264S) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with Anti MEK1(P264S) monoclonal antibody (Cat. #26266). |
| Immunofluorescence: |
| Immunofluorescence of cells expressing MEK1 proteins with Anti MEK1(P264S) antibody. HEK293T cells were transfected with pCDNA3-GFP MEK1(WT) plasmid (left column) or pCDNA3-GFP MEK1(P264S) plasmid (right column), then fixed and stained with Anti MEK1(P264S) monoclonal antibody (Cat. #26266). |
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