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武漢紐斯特生物科技有限公司
武漢紐斯特生物科技有限公司
Anti EGFR(vIII) Mouse Monoclonal Antibody
EGFR(vIII) [26176]
[100 µL]
| Catalog Number: 26176 |
| Gene Symbol: EGFR/HER1/ERBB1 |
| Description: Anti EGFR(vIII) Mouse Monoclonal Antibody |
| Background: EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (NSCLC). |
| Immunogen: A synthetic peptide from the internal region of EGFR which includes the mutation of vIII, human origin. |
| Applications: ELISA, WB, IF, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:1000-1:5000 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ ａnd Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes EGFR(vIII), but not wild type EGFR from vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Download Datasheet: |
| Western blot: |
| Western blot analysis of recombinant EGFR(vIII) and wildtype proteins. Purified His-tagged EGFR(vIII) protein (partial, lane 2) and corresponding wild type protein (lane 1) were blotted with anti-EGFR(vIII) mouse monoclonal antibody (Cat. # 26176). |
| Immunofluorescence: |
| Immunofluorescence of cells expressing EGFR proteins with anti EGFR(vIII) antibody. HEK293T cells were transfected with pCDNA3-GFP EGFR(WT) plasmid (left column) or pCDNA3-GFP EGFR(vIII) plasmid (right column), then fixed and stained with anti EGFR(vIII) monoclonal antibody (Cat. #26176). |
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| Publications: |
| 1. Tumor Biology. October 2014 |
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