B-RAF(G469E)[26196][100ul]CatalogNumber:26196GeneSymbol:BRAF,BRAF1,RAFB1Description:AntiBRAF(G469E)MouseMonoclonalAntibodyBackground:BRAFbelongstoafamilyofserine-threonineproteinkinases

詳細介紹

B-RAF(G469E) [26196]
[100ul]

Catalog Number: 26196

Gene Symbol: BRAF, BRAF1, RAFB1

Description: Anti BRAF(G469E) Mouse Monoclonal Antibody

Background: BRAF belongs to a family of serine-threonine protein kinases. As a part of a signaling pathway known as the the RAS/MAPK pathway, it plays an important part in many cellular processes. The processes include cell proliferation, differentiation and transcriptional regulation. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers.

Immunogen: A synthetic peptide from the internal region of BRAF which includes the mutation of G469E, human origin.

Applications: ELISA, WB, IHC

Recommended Dilutions:
ELISA: 1:1000-1:5000
WB: 1:500-1:1000
IHC: 1:50-1:100

Concentration: 1mg/ml

Host: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype:

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg²⁺ ａnd Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes G469E mutant, but not wild-type BRAF of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Download Datasheet:

Western blot:

Western blot analysis of recombinant BRAF (G469E) and wildtype proteins. Purified His-tagged BRAF (G469E) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with Anti BRAF(G469E) monoclonal antibody (Cat. #26196).

Immunofluorescence:

Immunofluorescence of cells expressing BRAF proteins with Anti BRAF(G469E) antibody. HEK293T cells were transfected with pCDNA3-GFP BRAF(WT) plasmid (left column) or pCDNA3-GFP-BRAF (G469E) plasmid (right column), then fixed and stained with Anti BRAF(G469E) monoclonal antibody (Cat. #26196).

Related Products
Catalog#	Name	Size	Price
	Anti BRaf Rabbit Polyclonal Antibody	100 μL	￥2600
	Anti BRaf Mouse Monoclonal Antibody	100 μL	￥2800
	Anti BRaf(N581S) Mouse Monoclonal Antibody	100 μL	￥4800
	Anti BRaf(V600E) Mouse Monoclonal Antibody	100 μL	￥4800
	Anti BRaf (V600K) Mouse Monoclonal Antibody	100 μL	￥4800
	Anti BRaf(V600D) Mouse Monoclonal Antibody	100 μL	￥4800
	Anti BRaf(V600R) Mouse Monoclonal Antibody	100 μL	￥4800
	Anti BRaf(G469E) Mouse Monoclonal Antibody	100 μL	￥4800