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武漢紐斯特生物科技有限公司
武漢紐斯特生物科技有限公司
Anti-EGFR (L858Q) Mouse Monoclonal Antibody
EGFR (L858Q)
Catalog Number: 26221
Gene Symbol: EGFR/HER1/ERBB1
Description: Anti-EGFR (L858Q) Mouse Monoclonal Antibody
| Background: EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (LNCR). Immunogen: A synthetic peptide from the internal region of EGFR which includes the mutation of L858Q, human origin. Tested applications: ELISA, WB, IF, IHC Recommended dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IHC: 1:50-1:100 Concentration: 1 mg/ml Host: Mouse Clonality: Monoclonal Purity: Purified from ascites Format: Liquid Storage buffer: Preservative: no Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol Species Reactivity: Recognizes EGFR (L858Q), but not wild type EGFR protein from vertebrates. Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles. | Western blot: Western blot analysis of recombinant EGFR (L858Q) and wildtype proteins. Purified His-tagged EGFR (L858Q) protein (aa702-987, lane 1) and corresponding wild-type protein (aa702-987, lane 2) were blotted with anti-EGFR (L858Q) mouse antibody (Cat. #26221). |
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