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武漢紐斯特生物科技有限公司
武漢紐斯特生物科技有限公司
IDH1(R132S)[26160][100µL]CatalogNumber:26160GeneSymbol:IDH1
IDH1(R132S) [26160]
[100 µL]
| Catalog Number: 26160 |
| Gene Symbol: IDH1; IDCD; IDH; IDP; IDPC; PICD |
| Description: Anti IDH1(R132S) Mouse Monoclonal Antibody |
| Background: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations affecting Arg-132 are tissue-specific, and suggest that this residue plays a unique role in the development of high-grade gliomas. Mutations of Arg-132 to Cys, His, Leu or Ser abolish magnesium binding and abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R-2-hydroxyglutarate. Elevated levels of R-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors. |
| Immunogen: A synthetic peptide from the internal region of IDH1 which includes the mutation of R132S, human origin. |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ ａnd Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes IDH1(R132S) mutated prteein, but not wild-type IDH1 of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Download Datasheet: |
| Western blot: |
| Western blot analysis of recombinant IDH1(R132S) and wild-type proteins. Purified His-tagged IDH1(R132S) protein (lane 2) and wild-type protein (lane 1) were blotted with Anti IDH1(R132S) mouse antibody (Cat. #26160). |
| Immunofluorescence: |
| Immunofluorescence of cells expressing IDH1 proteins with Anti IDH1(R132S) antibody. HEK293T cells were transfected with pCDNA3-GFP IDH1(WT) plasmid (left column) or pCDNA3-GFP IDH1(R132S) plasmid (right column), then fixed and stained with Anti IDH1(R132S) monoclonal antibody (Cat. #26160). |
| Related Products | |||
| Catalog# | Name | Size | Price |
| Anti IDH1 Mouse Monoclonal Antibody | 100 μL | ￥4800 | |
| Anti IDH1(R132P) Mouse Monoclonal Antibody | 100 μL | ￥4,800 | |
| Anti IDH1(R132S) Mouse Monoclonal Antibody | 100 μL | ￥4,800 | |
| Anti IDH1(R132H) Mouse Monoclonal Antibody | 100 μL | ￥4800 | |
| Publications: |
| 1. Brain Tumor Pathology. January 2015, Volume 32, Issue 1, pp 3-11 |
| 2. Nature 513, 110–114 (04 September 2014) |
| 3. Journal of Neurochemistry. Volume 132, Issue 2, pages 183–193, January 2015 |
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