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Anti ATM(F858L) Mouse Monoclonal Antibody

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Anti ATM(F858L) Mouse Monoclonal Antibody

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ATM(F858L) [26141]
[100 µL]

Catalog Number: 26141

Gene Symbol: ATM

Description: Anti ATM(F858L) Mouse Monoclonal Antibody

Background: Ataxia telangiectasia mutated (ATM) is a serine/threonine protein kinase that is recruited and activated by DNA double-strａnd breaks. It phosphorylates several key proteins that initiate activation of the DNA damage checkpoint, leading to cell cycle arrest, DNA repair or apoptosis. Activity of ATM protein is under tight control, and mutation of ATM can cause disease such as Ataxia telangiectasia (AT) and cancers.

Immunogen: A synthetic peptide from the internal region of ATM which includes the mutation of F858L, human origin.

Applications: ELISA, WB, IHC

Recommended Dilutions:
ELISA: 1:1000-1:5000
WB: 1:100-1:1000
IHC: 1:50-1:100

Concentration: 1 mg/ml

Host: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype:

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg²⁺ ａnd Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes ATM(F858L), but not wild type ATM protein from vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Download Datasheet:

Western blot:

Western blot analysis of recombinant ATM(F858L) and wildtype proteins. Purified His-tagged ATM(F858L) protein (amino acids 2400-3056, lane 2) and corresponding wild type protein (lane 1) were blotted with Anti ATM(F858L) mouse monoclonal antibody (Cat. #26141).

Immunofluorescence:

Immunofluorescence of cells expressing ATM(F858L) protein with anti-ATM(F858L) antibody. HEK293T cells were transfected with pCDNA3-GFP ATM(WT) plasmid (left column) or pCDNA3-GFP ATM(F858L) plasmid (right column), then fixed and stained with Anti ATM(F858L) monoclonal antibody (Cat. #26141).

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