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Anti AKT1(E49K) Mouse Monoclonal Antibody

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AKT1(E49K)[26137][100ul]CatalogNumber:26137GeneSymbol:AKT1

詳細介紹

AKT1(E49K) [26137]
[100ul]

Catalog Number: 26137

Gene Symbol: AKT1; AKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA

Description: Anti AKT1(E49K) Mouse Monoclonal Antibody

Background: AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinases, which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. Its function is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. There are evidences that mutation or abnormal expression in AKT1 is associated with cancers.

Immunogen: A synthetic peptide from the internal region of AKT1 which includes the mutation of E49K, human origin.

Applications: ELISA, WB, IHC

Recommended Dilutions:
ELISA: 1:1000-1:5000
WB: 1:500-1:2000
IHC: 1:50-1:100

Concentration: 1 mg/ml

Host: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype:

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg²⁺ ａnd Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes E49K mutant, but not wild-type AKT1 of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Download Datasheet:

Western blot:

Western blot analysis of recombinant AKT1(E49K) and wildtype proteins. Purified His-tagged AKT1(E49K) protein (lane 2) and corresponding wild type protein (lane 1) were blotted with Anti AKT1(E49K) monoclonal antibody (Cat. #26137).

Immunofluorescence:

Immunofluorescence of cells expressing AKT1 proteins with Anti AKT1(E49K) antibody. HEK293T cells were transfected with pCDNA3-GFP AKT1(WT) plasmid (left column) or pCDNA3-GFP AKT1(E49K) plasmid (right column), then fixed and stained with Anti AKT1(E49K) monoclonal antibody (Cat. #26137).