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武漢紐斯特生物科技有限公司
武漢紐斯特生物科技有限公司
EGFR(G719S)[26083][100µL]CatalogNumber:26083GeneSymbol:EGFRDescription:AntiEGFR(G719S)MouseMonoclonalAntibodyBackground:EGFRisareceptortyrosinekinaseforligandsoftheEGFfamily
EGFR(G719S) [26083]
[100 µL]
| Catalog Number: 26083 |
| Gene Symbol: EGFR |
| Description: Anti EGFR(G719S) Mouse Monoclonal Antibody |
| Background: EGFR is a receptor tyrosine kinase for ligands of the EGF family. EGFR activates several signaling cascades to convert extracellular cues into appropriate cellular responses. Defects in EGFR are associated with cancers such as lung cancer. |
| Immunogen: A synthetic peptide from the internal region of human EGFR which includes the mutation of G719S. |
| Applications: ELISA, WB, IHC, IF |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:1000-1:2000 IHC: 1:50-1:1000 IF: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host: mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG2b |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ ａnd Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes EGFR(G719S), but not wild type EGFR protein of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles. |
| Download Datasheet: |
| Western blot: |
| Western blot analysis of recombinant EGFR(G719S) and wild-type EGFR proteins. Purified his-tagged EGFR(G719S) protein (aa 702-987) (Cat. #10214) and wild-type EGFR protein (aa 702-987) (Cat. #10210) were blotted with anti EGFR(G719S) mouse antibody (Cat. #26083). |
| Immunofluorescence: |
| Immunofluorescence of cells expressing EGFR proteins with anti EGFR(G719S) antibody. HEK293T cells were transfected with pCDNA3-GFP EGFR(WT) plasmid (left column) or pCDNA3-GFP EGFR(G719S) plasmid (right column), then fixed and stained with anti EGFR(G719S) monoclonal antibody (Cat. #26083). |
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| Publications: |
| 1. Tumor Biology. October 2014 |
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