TP53(R175H)mono[26072][100µL]CatalogNumber:26072GeneSymbol:TP53(R175H)Description:AntiTP53(R175H)MouseMonoclonalAntibodyBackground:TP53belongstothep53proteinsfamily

詳細介紹

TP53(R175H) mono [26072]
[100 µL]

Catalog Number: 26072

Gene Symbol: TP53(R175H)

Description: Anti TP53(R175H) Mouse Monoclonal Antibody

Background: TP53 belongs to the p53 proteins family. It is ubiquitously expressed, acts as a tumor suppressor in many tumor types and induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Defects in TP53 are a cause of esophageal cancer (ESCR), lung cancer (LNCR), adrenocortical carcinoma (ADCC), choroid plexus papilloma (CPLPA) and so on.

Immunogen: A synthetic peptide from the internal region of TP53 which includes the mutation of R175H, human origin

Applications: ELISA, WB, IHC

Recommended Dilutions:
ELISA: 1:1000-1:5000
WB: 1:1000-1:1000
IHC: 1:50-1:100

Concentration: 1 mg/ml

Host: mouse

Format: Liquid

Clonality: Monoclonal

Isotype:

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg²⁺ ａnd Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Anti TP53(R175H) antibody recognizes TP53(R175H) of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles.

Download Datasheet:

Western blot:

	Western blot analysis of recombinant TP53(R175H) and wildtype proteins. Purified His-tagged TP53(R175H) protein(Cat.#10207) and wildtype protein(Cat.#10202) were blotted with Anti TP53(R175H) monoclonal antibody(Cat.# 26072).
Immunofluorescence:

Immunofluorescence of cells expressing TP53 proteins with anti TP53(R175H) antibody. HEK293T cells were transfected with pCDNA3-GFP TP53(WT) plasmid (left column) or pCDNA3-GFP TP53(R175H) plasmid (right column), then fixed and stained with anti- TP53(R175H) monoclonal antibody (Cat. # 26072).

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