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武漢紐斯特生物科技有限公司
武漢紐斯特生物科技有限公司
BRaf(V600E)[26039][100µL]CatalogNumber:26039GeneSymbol:BRafDescription:AntiBRaf(V600E)MouseMonoclonalAntibodyBackground:BRafisamemberoftheRaffamilyofSer/Thrproteinkinases
BRaf(V600E) [26039]
[100 µL]
| Catalog Number: 26039 |
| Gene Symbol: BRaf |
| Description: Anti BRaf(V600E) Mouse Monoclonal Antibody |
| Background: BRaf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase signaling pathway. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers |
| Immunogen: A synthetic peptide from the internal region of BRaf(V600E), human origin. |
| Applications: ELISA, WB, IHC, IP |
| Recommended Dilutions: ELISA: 1:100-1:5000 WB: 1:100-1:2000 IHC: 1:100-1:1000 |
| Concentration: 1 mg/ml |
| Host: mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG2b |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ ａnd Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes BRaf(V600E), but not wild type BRaf proteins from vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Download Datasheet: |
| Western blot: |
| Western blot analysis of recombinant Braf proteins with anti-BRaf(V600E) antibody. Purified His - tagged BRaf(V600E ) ?amino acids 513-693)(lane1) ? corresponding wild type protein(lane 2), His - tagged BRaf(V600D ) (lane 3) , His - tagged BRaf(V600A ) (lane 4) , His - tagged BRaf(V600G ) (lane 5) , His - tagged BRaf(V600K ) (lane 6), His - tagged BRaf(V600R ) (lane 7), and His - tagged BRaf( V600M ) (lane 8) were blotted with anti BRaf(V600E) monoclonal antibody (Cat # 26039) . |
| Immunofluorescence: |
| Immunofluorescence of cell expressing BRaf protein with anti BRaf(V600E) antibody . HEK293T cells were transfected with pCDNA3-GFP BRaf(V600E )plasmid,pCDNA3-GFP BRaf( WT )plasmid, pCDNA3-GFP BRaf(V600K )plasmid,pCDNA3-GFP BRaf(V600R )plasmid, pCDNA3-GFP- BRaf(V600A )plasmid,pCDNA3-GFP BRaf(V600D ) plasmid, pCDNA3-GFP-BRaf(V600M)plasmid, or pCDNA3-GFP-B-Raf(V600G)plasmid, then fixed and stained with anti BRaf(V600E) monoclonal antibody (Cat. # 26039). |
| Immunohistochemistry: |
| Immunohistochemical analysis of paraffin-embedded human melanoma tissue with anti BRaf(V600E) monoclonal antibody (Cat. # 26039). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:100) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (dilution 1:50) was used as secondary antibody. |
Immunohistochemical analysis of paraffin embedded Thyroid Carcinoma tissue -with anti BRaf(V600E) monoclonal antibody (Cat. # 26039). Tissue samples were fixed with |
Immunohistochemical analysis of paraffin embedded Thyroid Carcinoma tissue -with anti BRaf(V600E) monoclonal antibody (Cat. # 26039). Tissue samples were fixed with paraffin. Samples were then incubated with primary antibody (1:100) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (dilution 1:50) was used as secondary antibody. Allele specific PCR validated to be Negative for BRAF V600E. |
| Related Products | |||
| Catalog# | Name | Size | Price |
| Anti BRaf Rabbit Polyclonal Antibody | 100 μL | ￥2600 | |
| Anti BRaf Mouse Monoclonal Antibody | 100 μL | ￥2800 | |
| Anti BRaf(N581S) Mouse Monoclonal Antibody | 100 μL | ￥4800 | |
| Anti BRaf(V600E) Mouse Monoclonal Antibody | 100 μL | ￥4800 | |
| Anti BRaf(V600K) Mouse Monoclonal Antibody | 100 μL | ￥4800 | |
| Anti BRaf(V600D) Mouse Monoclonal Antibody | 100 μL | ￥4800 | |
| Anti BRaf(V600R) Mouse Monoclonal Antibody | 100 μL | ￥4800 | |
| Anti BRaf(G469E) Mouse Monoclonal Antibody | 100 μL | ￥4800 | |
| Anti BRaf(V600A) Mouse Monoclonal Antibody | 100 μL | ￥4800 | |
| Anti BRAF(V600M) Mouse Monoclonal Antibody | 100 μL | ￥4800 |
| Publications: |
| 1. Mod Pathol. 2013 Mar;26(3):414-20. |
| 2. Tumor Biology. June 2014, Volume 35, Issue 6, pp 5727-5733 |
| 3. human pathology. February 2015Volume 46, Issue 2, Pages 169–175 |
| 4. human pathology. November 2013Volume 44, Issue 11, Pages 2563–2570 |
| 5. Seminars in Diagnostic Pathology |
6. Oncotarget. 2016 Aug 2; 7(31): 49597–49610. |
7. Sci Rep. 2019; 9: 1032. |
8. Oncotarget. 2017 Aug 29; 8(35): 58021–58036 |
9. Gastroenterology Res. 2018 Oct; 11(5): 355–360 |
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