上海研盟生物科技有限公司Anti-MNK2抗體*，主要應用于WB、IHC、IF、ELISA、流式細胞術等實驗中。說明書隨貨發送，您也可以直接我司在線客服索取。客服
英文名稱：Anti-MNK2 antibody
中文名稱：MAP激酶相互作用*/*激酶2抗體
MAP激酶相互作用*/*激酶2抗體保存條件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
MAP激酶相互作用*/*激酶2抗體,Anti-MNK2抗體抗原修復方法：
方法1：沸水浴修復，將盛有修復液和玻片的燒杯置于沸水浴環境，保持外部沸騰狀態15min，自然冷卻至室溫。
方法2：微波修復，將盛有修復液和玻片的燒杯置于微波爐中，高火5min，停火3min，中火5min，自然冷卻至室溫。
方法3：高壓修復，修復液加入高壓鍋加熱至沸騰，放入玻片，封蓋加壓持續加熱至噴氣時開始計時修復2min，自然冷卻至室溫。
方法4：使用*修復，將*修復液加入到組織上，37℃，消化30min
★研盟生物★MAP激酶相互作用*/*激酶2抗體,Anti-MNK2抗體
Western Blotting 實驗步驟概述：
1、 組織取材：組織塊稱重
2、 利用液氮、研缽粉碎組織塊
3、 加入RIPA緩沖液（每克組織3ml RIPA），PMSF（每克組織30μl，10 mg/ml PMSF），
利用Polytron進一步勻漿（15,000轉/分*1分鐘）維持4℃
4、 加入PMSF（每克組織30μl，10 mg/ml PMSF），冰上孵育30分鐘
5、 移入離心管4℃，約20,000g（約15,000轉）15分鐘
6、 上清液為細胞裂解液可分裝-20℃保存
7、 進行Bradford比色法測定蛋白質濃度
8、 取相同質量的細胞裂解液（體積*蛋白質濃度），并加等體積的2×電泳加樣緩沖液
9、 沸水浴中3分鐘
10、上樣
11、電泳（濃縮膠20mA，分離膠35mA）
12、電轉膜儀轉膜（100mA 40分鐘）
13、膜用麗春紅染色，膠用考馬斯亮藍染色
14、Westernblot 試劑盒顯色
15、分析比較記錄
★研盟生物 ★合作： ★客服
相關標記抗體：HRP標記抗體,Biotin標記抗體,Gold標記抗體,RBITC標記抗體,AP標記抗體,FITC標記抗體,Cy3標記抗體,Cy5標記抗體,Cy5.5標記抗體,Cy7標記抗體,PE標記抗體,PE-Cy3標記抗體,PE-Cy5標記抗體,PE-Cy5.5標記抗體,PE-Cy7標記抗體,APC標記抗體,Alexa Fluor 350標記抗體,Alexa Fluor 488標記抗體,Alexa Fluor 555標記抗體,Alexa Fluor 647標記抗體
簡單介紹：
產品別名：G protein coupled receptor kinase 7; GPRK7; MAP kinase interacting serine threonine kinase 2; MAP kinase interacting serine threonine kinase; MAP kinase interacting serine threonine protein kinase 2; MAP kinase signal integrating kinase 2; MAP kinase signal-integrating kinase 2; MAP kinase-interacting serine/threonine-protein kinase 2; MAPK interacting kinase 2; MAPK signal integrating kinase 2; Mitogen activated protein kinase interacting serine threonine kinase 2; Mknk2; MKNK2_HUMAN; Mnk2; Putative map kinase interacting kinase.
抗體來源：Rabbit or Mouse
保質期:1年
克隆類型：Polyclonal or monoclonal
性 狀：Lyophilized or Liquid
濃 度：1mg/1ml
背景介紹：This gene encodes a member of the calcium/calmodulin-dependent protein kinases (CAMK) Ser/Thr protein kinase family, which belongs to the protein kinase superfamily. This protein contains conserved DLG (asp-leu-gly) and ENIL (glu-asn-ile-leu) motifs, and an N-terminal polybasic region which binds importin A and the translation factor scaffold protein eukaryotic initiation factor 4G (eIF4G). This protein is one of the downstream kinases activated by mitogen-activated protein (MAP) kinases. It phosphorylates the eukaryotic initiation factor 4E (eIF4E), thus playing important roles in the initiation of mRNA translation, oncogenic transformation and malignant cell proliferation. In addition to eIF4E, this protein also interacts with von Hippel-Lindau tumor suppressor (VHL), ring-box 1 (Rbx1) and Cullin2 (Cul2), which are all components of the CBC(VHL) ubiquitin ligase E3 complex. Multiple alternatively spliced transcript variants have been found, but the full-length nature and biological activity of only two variants are determined. These two variants encode distinct isoforms which differ in activity and regulation, and in subcellular localization.
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