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上海研盟生物科技有限公司
上海研盟生物科技有限公司
上海研盟生物科技有限公司“MMP-26抗體-基質金屬蛋白酶-26抗體"抗體規格0.1ml、0.2ml、1ml，抗體產品有效期一年，出現任何質量問題或是出不了實驗結果均可免費退換。：、
MMP-26抗體-基質金屬蛋白酶-26抗體免疫組化操作步驟
（一）、儀器設備
1、 18cm不銹鋼高壓鍋或電爐或用微波爐
2、 水浴鍋
（二）、試 劑
1、 PBS緩沖液（pH7.2―7.4）
2、 0.01mol/L檸檬酸鈉緩沖液（CB,pH6.0,1000ml）
3、 3%甲醇―H2O2溶液：用30% H2O2 和80%甲醇溶液配制
4、 風裱劑：
a. 甘油和0.5mmol/L碳酸鹽緩沖液（pH9.0–9.5）等量混合
b. 油和TBS（PBS）配制
5、 TBS/PBS pH9.0–9.5，適用于熒光纖維鏡標本；pH7.0-7.4適合光學纖維標本
（三）、操作流程
1、 脫蠟和水化：脫蠟前應將組織芯片在室溫中放置60分鐘或60℃恒溫箱中烘烤20分鐘
a. 組織芯片置于二甲苯中浸泡10分鐘，更換二甲苯后在浸泡10分鐘
b. 無水乙醇中浸泡五分鐘
c. 95%乙醇中浸泡五分鐘
d. 75%乙醇中浸泡五分鐘
2、 抗原修復：用于福爾馬林固定的石蠟包埋組織芯片
【中文名稱】：基質金屬蛋白酶-26抗體
【英文名稱】：Anti-MMP-26
【產品編號】：BYK-1377R
【規 格】：0.1ml/0.2ml
【相關標記】：Alexa Fluor 350、Alexa Fluor 488、Alexa Fluor 555、Alexa Fluor 647、AP、APC、Biotin、Cy3、Cy5、Cy5.5、Cy7、FITC、Gold、HRP、PE、PE-Cy3、PE-CY5、PE-CY5.5、PE-CY7、RBITC.
【濃 度】：1mg/1ml
【抗體來源】：Rabbit or Mouse or Goat
【克隆類型】：polyclonal or monoclonal
【產品類型】：一抗
【產品應用】：可以用于做石蠟切片免疫組化，冰凍切片免疫組化，Elisa，WB，免疫熒光、流式細胞術等實驗。
抗體修復方式
修復液：0.01M 枸櫞酸（pH6.0）或者0.05M EDTA（pH8.0）
MMP-26抗體-基質金屬蛋白酶-26抗體產品介紹：Matrix metalloproteinase 26 preprotein; gelatinase A; 70kD type IV collagenase; gelatinase neutrophil. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes as well as in disease processes. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP26 degrades type IV collagen, the major structural component of basement membranes. The enzyme plays a role in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response.
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases.
MMP26, also known as Matrilysin 2, was first cloned from human fetal cells, and identified as an MMP most closely related to MMP7 (Matrilysin 1). The homology between MMP7 and MMP26 is low (only 38% identical), thus the functions are unlikely to be similar. Homology is much higher (48% identical) for the comparable region of MMP12, but MMP26 appears to have broader substrate specificity than does MMP12. MMP26, like MMP7, lacks the hemopexin domain common to the other MMPs, but contains a Propeptide domain, cysteine switch activation site, followed by a catalytic domain, and a short vestige of the hinge region. MMP26 is apparently not glycosylated, and is a secreted MMP. Tissue analysis shows MMP26 most strongly in placenta and uterus, but also in kidney cells, lung cells, lymphocytes and lung or endometrial carcinoma cells. MMP26 is proteolytically active, cleaving casein in zymograms, and gelatin, a1PI, fibrinogen, fibronectin, vitronectin, type IV collagen, and apparently activating MMP9. The proteolytic activity was blocked by TIMP1 and TIMP2. MMP26 does not appear to be produced by most normal quiescent cells, but treatment of many cell types with the phorbol ester TPA, or IL1 stimulates production of MMP26.
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英文別名：MMP26_HUMAN; Endometase; Matrilysin 2; MMP26; Matrix Metalloproteinase 26; MMP 26.
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