儲(chǔ) 存 液: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
保存條件: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Anti-Neurexin 2抗體,軸突蛋白2抗體產(chǎn)品介紹:This gene encodes a member of the neurexin gene family. The products of these genes function as cell adhesion molecules and receptors in the vertebrate nervous system. These genes utilize two promoters. The majority of transcripts are produced from the upstream promoter and encode alpha-neurexin isoforms while a smaller number of transcripts are produced from the downstream promoter and encode beta-neuresin isoforms. The alpha-neurexins contain epidermal growth factor-like (EGF-like) sequences and laminin G domains, and have been shown to interact with neurexophilins. The beta-neurexins lack EGF-like sequences and contain fewer laminin G domains than alpha-neurexins. Alternative splicing and the use of alternative promoters may generate thousands of transcript variants (PMID: 12036300, PMID: 11944992).
免疫組化操作流程 (一) 儀器設(shè)備 1. 18cm不銹鋼高壓鍋或電爐或用微波爐. 2. 水浴鍋 (二) 試 劑 1. PBS緩沖液(ph7.2―7.4):NaC137mmol/L,KCl 2.7mmol/L ,Na2HPO4 4.3mmol/L, KH2PO4 1.4mmol/L. 2.0.01mol/L檸檬酸鈉緩沖液(CB,ph6.0,1000ml):檸檬酸三鈉3g,檸檬酸0.4g。 3.0.5mol/L EDTA緩沖液(ph8.0):700ml水中溶解186.1g EDTA& 8226;2H2O,用10mmol/L NaOH調(diào)至ph8.0,加水至1000ml. 4. 1mol/L的TBS緩沖液(ph8.0):在800ml水中溶解121gTris堿,用1N的HCl調(diào)至ph8.0,加水1000ml。 5. 酶消化液:a. 0.1%*:用0.1%CaCl 12(ph7.8)配制。b.0.4%*液:用0.1N的HCl配制。 6. 3%甲醇―H2O2溶液:用30%H2O2和80%甲醇溶液配制 7. 風(fēng)裱劑:a. 甘油和0.5mmol/L碳酸鹽緩沖液(ph9.0–9.5)等量混合 b 油和TBS(PBS)配制 8.TBS/PBS PH9.0–9.5,適用于熒光纖維鏡標(biāo)本;ph7.0-7.4適合光學(xué)纖維標(biāo)本。 (三) 操作流程 1 脫蠟和水化:脫蠟前應(yīng)將組織芯片在室溫中放置60分鐘或60℃恒溫箱中烘烤20分鐘。 a 組織芯片置于二甲苯中浸泡10分鐘,更換二甲苯后在浸泡10分鐘 b 無水乙醇中浸泡五分鐘 c 95%乙醇中浸泡五分鐘 d 75%乙醇中浸泡五分鐘 2 抗原修復(fù):用于福爾馬林固定的石蠟包埋組織芯片: A 抗原熱修復(fù) a 高壓熱修復(fù) 在沸水中加入EDTA(ph8.0)或0.01m*緩沖溶液(ph6.0).蓋上不銹鋼鍋蓋,但不能鎖定。將玻片置于金屬染色加上,緩慢加壓,是玻片在緩沖液中浸泡五分鐘,然后將蓋子鎖定,小閥門將會(huì)升起來。10分鐘后除去熱源,置入涼水中,當(dāng)小閥門沉下去后打開蓋子。此方法適用于較難檢測(cè)或核抗原的抗原修復(fù)。 b 沸熱修復(fù) 電爐或水浴鍋加熱0.01*緩沖液(ph6.0)至95℃左右,放入組織芯片加熱10-15分鐘。 C 微波爐加熱 在微波爐里加熱0.01*緩沖液(ph6.0)至沸騰后將組織芯片放入,斷電,間隔5-10分鐘,反復(fù)1-2次。適用的抗原: Bcl-2.Bax.AR.PR.C-fos.x-jum.z-kit.c-myc.E-cadherin. ChromograninA.Cyclin.ER.Heatshock.Protein.HPV.Ki-67.MDMZ.P53.P34.P15.P-glycoprotein.PKC.PCNA.ras.Rb.等 B 酶消化方法 常用0.1%*和0.4%*液。*使用前預(yù)熱37℃,消化時(shí)間約為5-30分鐘。適用于被固定遮蔽的抗原:包括 Collagen.GFAP. Complement.Cytokeratin.C-erB-2.LCA.LN.等
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