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上海研盟生物科技有限公司
上海研盟生物科技有限公司
“Anti-phospho-alpha B Crystallin （Ser59）抗體,αb晶體蛋白質抗體"2A抗體規格0.1ml、0.2ml、1ml，抗體產品有效期一年，質保三個月，質保期內出現任何質量問題或是出不了實驗結果均可免費退換：
上海研盟生物科技有限公司Anti-phospho-alpha B Crystallin （Ser59）抗體,αb晶體蛋白質抗體*，主要應用于WB、IHC、IF、ELISA、流式細胞術等實驗中。說明書隨貨發送，您也可以直接我司在線客服索取。客服
簡單介紹：
中文名稱：磷酸化熱休克蛋白β5/αb晶體蛋白質/α-晶體蛋白b鏈抗體
英文名稱：Anti-phospho-alpha B Crystallin (Ser59)
產品編號：byk-12463R
產品別名：alpha B Crystallin (phospho S59); alpha B Crystallin (phospho Ser59); p-alpha B Crystallin (S59); p-alpha B Crystallin (Ser59); AACRYA; Alpha B crystallin; Alpha crystallin B chain; Alpha crystallin B chain; Alpha(B) crystallin; Alpha(B)-crystallin; Alpha-crystallin B chain; CRYA2; CRYAB; CRYAB_HUMAN; Crystallin alpha B; Crystallin alpha polypeptide 2; CTPP 2; CTPP2; Heat shock 20 kD like protein; Heat shock protein beta 5; Heat shock protein beta-5; HSPB5; NY REN 27 antigen; Renal carcinoma antigen NY REN 27; Renal carcinoma antigen NY-REN-27; Rosenthal fiber component.
產品規格：0.1ml、0.2ml、1ml
產品用途：科研實驗
產品價格：請報價
說明書：請添加客服直接索取
抗體來源：該指標有兩種產品，一種是兔來源抗體，一種是鼠來源抗體
克隆類型：兔來源為多克隆抗體，鼠來源單克隆抗體
交叉反應：請索取說明書查看
性 狀：Lyophilized or Liquid
濃 度：1mg/1ml
亞 型:IgG
純化方法: affinity purified by Protein A
儲 存 液: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
保存條件: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Anti-phospho-alpha B Crystallin （Ser59）抗體,αb晶體蛋白質抗體產品介紹：Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy.
抗原修復，必須注意以下問題：
1、修復液的選擇。
0.01M枸櫞酸（pH6.0）：應用zui多的抗原修復液，適用于大多數的抗原，用該液修復后的抗原表達增強，抗原的定性和定位很理想，結果不錯;
0.05M EDTA（pH8.0）：應用較多的抗原修復液，修復能力較強，對于保存時間較長的切片或目的蛋白表達較弱的組織有很好的修復效果，需要控制好修復時間，否則容易出現較重的背景;
0.01M TBS（pH7.4）：中性修復液，適用于大多數抗原，對于核定位蛋白有較好的修復效果;
*：應用較多的抗原修復液，適用于大多數的抗原，使用溫度37℃，對于容易脫片的切片有很好的保護作用。
*：應用較多的抗原修復液，適用于大多數的抗原
2.抗原熱修復時應選擇的溫度。
據實驗認為溫度在92℃－98℃是合適的，尤其在95℃為，這是因為：（1）這種溫度未達沸騰，切片不容易脫離載玻片；（2）能夠解離和破壞與蛋白交聯的甲醛醛鍵等，處理時可以隨意選擇和確定抗原修復的作用時間。如果選擇高壓修復，因為溫度較高，時間不宜過長
3.抗原修復時有效溫度所需持續時間根據各單位的設備條件以及使用的儀器不同，抗原修復時在有效的溫度范圍內所持續的時間也不一樣。
4.抗原修復液必須遵循自然降溫規律，否則效果不好或達不到抗原修復的目的。
5.盡量使用足量的抗原修復液，防止切片干涸。
6.切片必須附貼牢固，否則發生掉片。
Anti-phospho-alpha B Crystallin （Ser59）抗體,αb晶體蛋白質抗體相關產品：
S-100A1 (S100 calcium-binding protein A1) 鈣結合蛋白S100A1抗體
S100A13(S100 Calcium Binding Protein A13 ) 鈣結合蛋白S100A13抗體
SAMDC/AMD1（S-adenosylmethionine decarboxylase） S-腺苷蛋氨酸脫羧酶抗體
Shrimp tropomyosin(South American shrimp allergen protein) 南美白對蝦過敏原蛋白（蝦原肌球蛋白）抗體
SAP-1/SynaptopBYKin/FRG4/SYP(Synapse associated protein- I)hu, mo, rat, dog, bov, chi, hoBYKe, pig 突觸相關蛋白-1/突觸素抗體
SABYK putative orflab polyprotein （SABYK coronavirus CUHK-W1）
SABYK S-protein 非典病毒表面蛋白S抗體
SATB1(special AT-rich sequence binding protein) 核基質結合區結合蛋白1抗體
SCF (Stem Cell Factor) 干細胞生長因子抗體
SCG3/SgIII(Secretogranin 3) 分泌粒蛋白Ⅲ抗體
SCN1A(sodium channel voltage-gated type I alpha) 電壓閥門鈉通道蛋白a1/癲癇相關蛋白抗體
SCN2A（sodium channel voltage-gated type II alpha） 電壓閥門鈉通道蛋白a2/癲癇相關蛋白抗體
SCN9A/NAV1.7(Sodium channel protein type 9 subunit alpha) 電壓開啟的鈉離子通道SCN9A抗體
SDF-1/CXCL12 (stromal cell derived factor-1) 基質細胞衍生因子－1抗體
CXCL14/BRAK(C-X-C motif chemokine 14) CXC趨化因子14抗體
CXCL16(CXC-chemokine ligand 16) CXC趨化因子16抗體
Secretin 分泌素抗體
Secretin receptor 分泌素受體抗體
SEL1L（Suppressor of lin-12-like Protein） SEL1L抗體
Selenoprotein W 硒蛋白W抗體
Sema3A (semaphorin 3A) 臂板蛋白3A抗體
Sema3F (semaphorin 3F) 臂板蛋白3F抗體
Sema4C (semaphorin 4C) 臂板蛋白4C抗體
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