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“Anti-5HT3D+5HT3E Receptor抗體,5-羥色胺受體3D+3E抗體"2A抗體規格0.1ml、0.2ml、1ml,抗體產品有效期一年,質保三個月,質保期內出現任何質量問題或是出不了實驗結果均可免費退換:
上海研盟生物科技有限公司Anti-5HT3D+5HT3E Receptor抗體,5-羥色胺受體3D+3E抗體*,主要應用于WB、IHC、IF、ELISA、流式細胞術等實驗中。說明書隨貨發送,您也可以直接我司在線客服索取。客服
簡單介紹:
中文名稱:5-羥色胺受體3D+3E抗體
英文名稱:Anti-5HT3D+5HT3E Receptor
產品編號:byk-12052R
產品別名:5 HT3 D; 5 HT3 E; 5 HT3c1; 5 HT3D; 5-HT3-D; 5-HT3D; 5 HT3E; 5 hydroxytryptamine (serotonin) receptor 3 family member D; 5-hydroxytryptamine receptor 3D; 5 hydroxytryptamine (serotonin) receptor 3 family member E; 5 hydroxytryptamine receptor 3 subunit D; 5 hydroxytryptamine receptor 3 subunit E; 5 hydroxytryptamine receptor 3D; 5 hydroxytryptamine receptor 3E; 5HT3D; HTR3D; HTR3E; MGC119636; MGC119637; MGC120035; MGC120036; MGC120037; Serotonin 5 HT 3D receptor; Serotonin receptor 3D; Serotonin receptor 3E; 5HT3D_HUMAN; 5HT3E_HUMAN.
產品規格:0.1ml、0.2ml、1ml
產品用途:科研實驗
產品價格:請報價
說明書:請添加客服直接索取
抗體來源:該指標有兩種產品,一種是兔來源抗體,一種是鼠來源抗體
克隆類型:兔來源為多克隆抗體,鼠來源單克隆抗體
交叉反應:請索取說明書查看
性 狀:Lyophilized or Liquid
濃 度:1mg/1ml
亞 型:IgG
純化方法: affinity purified by Protein A
儲 存 液: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
保存條件: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Anti-5HT3D+5HT3E Receptor抗體,5-羥色胺受體3D+3E抗體產品介紹:Serotonin is a monoamine neurotransmitter that is made in serotonergic neurons in the CNS (central nervous system) and is important in the regulation of mood, sleep, vomiting, sexuality and appetite. 5-HT3 (5-hydroxytryptamine-3) receptor is the only ligand-gated ion channel within the family of serotonin receptors. It is composed of five subunits consisting of SR-3A, SR-3B, HTR3C, HTR3D and HTR3E. HTR3D (5-hydroxytryptamine receptor 3D), also known as Serotonin receptor 3D, is a 454 amino acid multi-pass membrane protein that is one components of the pentaheteromeric complex that forms the 5-HT3 receptor. HTR3D must be co-expressed with SR-3A to form a functional 5-HT3 receptor complex on the plasma membrane. Until it is complexed with SR-3A, HTR3D is localized within the endoplasmic reticulum. Expression of HTR3D is restricted to kidney, colon and liver. There are three different isoforms of HTR3D that are expressed as a result of alternative splicing events.
抗原修復,必須注意以下問題:
1、修復液的選擇。
0.01M枸櫞酸(pH6.0):應用zui多的抗原修復液,適用于大多數的抗原,用該液修復后的抗原表達增強,抗原的定性和定位很理想,結果不錯;
0.05M EDTA(pH8.0):應用較多的抗原修復液,修復能力較強,對于保存時間較長的切片或目的蛋白表達較弱的組織有很好的修復效果,需要控制好修復時間,否則容易出現較重的背景;
0.01M TBS(pH7.4):中性修復液,適用于大多數抗原,對于核定位蛋白有較好的修復效果;
*:應用較多的抗原修復液,適用于大多數的抗原,使用溫度37℃,對于容易脫片的切片有很好的保護作用。
*:應用較多的抗原修復液,適用于大多數的抗原
2.抗原熱修復時應選擇的溫度。
據實驗認為溫度在92℃-98℃是合適的,尤其在95℃為,這是因為:(1)這種溫度未達沸騰,切片不容易脫離載玻片;(2)能夠解離和破壞與蛋白交聯的甲醛醛鍵等,處理時可以隨意選擇和確定抗原修復的作用時間。如果選擇高壓修復,因為溫度較高,時間不宜過長
3.抗原修復時有效溫度所需持續時間根據各單位的設備條件以及使用的儀器不同,抗原修復時在有效的溫度范圍內所持續的時間也不一樣。
4.抗原修復液必須遵循自然降溫規律,否則效果不好或達不到抗原修復的目的。
5.盡量使用足量的抗原修復液,防止切片干涸。
6.切片必須附貼牢固,否則發生掉片。
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