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“Anti-TSHR (CT)抗體,促甲狀腺素受體抗體(C端)"2A抗體規格0.1ml、0.2ml、1ml,抗體產品有效期一年,質保三個月,質保期內出現任何質量問題或是出不了實驗結果均可免費退換:
上海研盟生物科技有限公司Anti-TSHR (CT)抗體,促甲狀腺素受體抗體(C端))貨*,主要應用于WB、IHC、IF、ELISA、流式細胞術等實驗中。說明書隨貨發送,您也可以直接我司在線客服索取。客服
簡單介紹:
中文名稱:促甲狀腺素受體抗體(C端)
英文名稱:Anti-TSHR (CT)
產品編號:byk-0206R
產品別名:hTSHR I; hTSHRI; LGR 3; LGR3; MGC75129; Thyroid adenoma hyperfunctioning; Thyroid carcinoma with thyrotoxicosis; Thyroid Stimulating Hormone Receptor; Thyrotropin Receptor; Thyrotropin receptor I; TSH R; TSHR; Thyroid stimulating hormone receptor; TSHR_HUMAN. 促甲狀腺激素受體
產品規格:0.1ml、0.2ml、1ml
產品用途:科研實驗
產品價格:請報價
說明書:請添加客服直接索取
抗體來源:該指標有兩種產品,一種是兔來源抗體,一種是鼠來源抗體
克隆類型:兔來源為多克隆抗體,鼠來源單克隆抗體
交叉反應:請索取說明書查看
性 狀:Lyophilized or Liquid
濃 度:1mg/1ml
亞 型:IgG
純化方法: affinity purified by Protein A
儲 存 液: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
保存條件: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Anti-TSHR (CT)抗體,促甲狀腺素受體抗體(C端)產品介紹:The glycoprotein hormone receptor family consists of the luteinizing hormone receptor, the follicle-stimulating hormone receptor, and the thyroid stimulating hormone(TSH) receptor. TSH, which is released from the pituitary gland, binds to the TSH receptor on thyroid cells to control size and function of the thyroid gland (De Felice et al. 2004). The TSH receptor signals through Gs to elevate intracellular cAMP in the thyroid gland, which regulates iodide uptake, and transcription of thyroglobulin (Tg), thyroid peroxidase (TPO), and sodium-iodide symporter. The TSH receptor also signals Gq and phospholipase C to regulat iodide efflux, H2O2 production, and thyroglobulin iodination. Autoimmunity to the TSH receptor causes hyperthyroidism (Graves disease) or hypothyroidism (Hashimoto thyroiditis) when the autoantibodies function as agonists or antagonists, respectively, at the TSH receptor (Rapoport and McLachlan, 2001; Davies et al., 2002). Millipore’s cloned human TSH receptor-expressing cell line is made in the Chem-10 host, which supports high levels of recombinant TSH receptor expression on the cell surface and contains high levels of the promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between TSH and its ligands.
抗原修復,必須注意以下問題:
1、修復液的選擇。
0.01M枸櫞酸(pH6.0):應用zui多的抗原修復液,適用于大多數的抗原,用該液修復后的抗原表達增強,抗原的定性和定位很理想,結果不錯;
0.05M EDTA(pH8.0):應用較多的抗原修復液,修復能力較強,對于保存時間較長的切片或目的蛋白表達較弱的組織有很好的修復效果,需要控制好修復時間,否則容易出現較重的背景;
0.01M TBS(pH7.4):中性修復液,適用于大多數抗原,對于核定位蛋白有較好的修復效果;
*:應用較多的抗原修復液,適用于大多數的抗原,使用溫度37℃,對于容易脫片的切片有很好的保護作用。
*:應用較多的抗原修復液,適用于大多數的抗原
2.抗原熱修復時應選擇的溫度。
據實驗認為溫度在92℃-98℃是合適的,尤其在95℃為,這是因為:(1)這種溫度未達沸騰,切片不容易脫離載玻片;(2)能夠解離和破壞與蛋白交聯的甲醛醛鍵等,處理時可以隨意選擇和確定抗原修復的作用時間。如果選擇高壓修復,因為溫度較高,時間不宜過長
3.抗原修復時有效溫度所需持續時間根據各單位的設備條件以及使用的儀器不同,抗原修復時在有效的溫度范圍內所持續的時間也不一樣。
4.抗原修復液必須遵循自然降溫規律,否則效果不好或達不到抗原修復的目的。
5.盡量使用足量的抗原修復液,防止切片干涸。
6.切片必須附貼牢固,否則發生掉片。
Anti-TSHR (CT)抗體,促甲狀腺素受體抗體(C端)相關產品:
PRL-2(Protein tyrosine phosphatase type IV A protein 2) 肝再生磷酸酯酶2抗體
PH-4/P4H-TM(proline 4-YMdroxylase) *水解酶4抗體
PHD3 (Egln3; HIF-prolyl YMdroxylase 3;) *羥化酶抗體
PS-1 (Presenilin-1/S182)(NT) 早老素蛋白-1抗體
PS-1(Presenilin-1/S182)(CT) 早老素蛋白-1抗體
PS-1(Presenilin-1/S182)(NT) 早老素蛋白-1抗體
PSA (Prostate specific antigen) for conjugation and detection 鼠抗前列腺特異性抗原抗體
PSA (Prostate Specific Antigen) mouse monoclonal(use for coating) 鼠抗前列腺特異性抗原單克隆抗體(包被)
PSA (Prostate Specific Antigen) mouse monoclonal(use for detection) 鼠抗前列腺特異性抗原單克隆抗體(優質產品)
CPSA(complex edprostate specific antigen mouse monoclonal) 鼠抗復合前列腺特異性抗原單克隆抗體
PSAP/PAP(Prostate Specific Acidic Phosphatase) 前列腺酸性磷酸酶抗體
PSCA (Prostate Stem Cell gen) 抗前列腺干細胞抗原抗體
PSD-95 突觸后密度蛋白95抗體
PSGL-1/CD162(P-selectin glycoprotein ligand 1) P選擇素糖蛋白配體1抗體
PSMD9/Bridge-1(proteasome modulator 9) 蛋白酶調解因子9
P-selectin/CD62p P選擇素抗體
L-Selectin/CD62L (ELAM-1) L選擇素抗體
E-Selectin/CD62E E選擇素抗體
Patched/PTCH (Patched protein homolog 1) Hh信號轉導途徑膜蛋白受體抗體
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