Anti-Megalin抗體,糖蛋白gp330抗體
英文介紹:The protein encoded by this gene, low density lipoprotein-related protein 2 (LRP2) or megalin, is a multi-ligand endocytic receptor that is expressed in many different tissues but primarily in absorptive epithilial tissues such as the kidney. This glycoprotein has a large amino-terminal extracellular domain, a single transmembrane domain, and a short carboxy-terminal cytoplasmic tail. The extracellular ligand-binding-domains bind diverse macromolecules including albumin, apolipoproteins B and E, and lipoprotein lipase. The LRP2 protein is critical for the reuptake of numerous ligands, including lipoproteins, sterols, vitamin-binding proteins, and hormones. This protein also has a role in cell-signaling; extracellular ligands include parathyroid horomones and the morphogen sonic hedgehog while cytosolic ligands include MAP kinase scaffold proteins and JNK interacting proteins. Recycling of this membrane receptor is regulated by phosphorylation of its cytoplasmic domain. Mutations in this gene cause Donnai-Barrow syndrome (DBS) and facio-oculoacoustico-renal syndrome (FOAR).
此產(chǎn)品本公司有標記的一抗出售,相關(guān)標記有:Alexa Fluor 350 標記、Alexa Fluor 488 標記、Alexa Fluor 555 標記、Alexa Fluor 647 標記、AP標記、APC標記、Biotin標記、Cy3標記、Cy5標記、Cy5.5標記、Cy7標記、FITC標記、Gold標記、HRP標記、PE標記、PE-Cy3標記、PE-CY5標記、PE-CY5.5標記、PE-CY7標記、RBITC標記
注意事項:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Anti-Megalin抗體,糖蛋白gp330抗體轉(zhuǎn)膜(Transfer):
我們*在Western實驗中選用PVDF膜。硝酸纖維素膜(NC膜)或PVDF膜(二氟化樹脂膜膜孔徑:0.45um)也可以使用,但硝酸纖維素膜比較脆,在操作過程中特別是用鑷子夾取等過程中容易裂開。膜的使用請參考生產(chǎn)商的*使用步驟。
通常如果使用Bio-Rad的標準濕式轉(zhuǎn)膜裝置,可以設定轉(zhuǎn)膜電壓120V,轉(zhuǎn)膜時間為30-60分鐘。具體的轉(zhuǎn)膜時間要根據(jù)目的蛋白的大小而定,目的蛋白的分子量越大,需要的轉(zhuǎn)膜時間越長,目的蛋白的分子量越小,需要的轉(zhuǎn)膜時間越短。
在轉(zhuǎn)膜過程中,特別是高電流快速轉(zhuǎn)膜時,通常會有非常嚴重的發(fā)熱現(xiàn)象,把轉(zhuǎn)膜槽放置在冰浴中進行轉(zhuǎn)膜。轉(zhuǎn)膜的效果可以觀察所使用的預染蛋白質(zhì)分子量標準,通常分子量zui大的1-2條帶較難全部轉(zhuǎn)到膜上。轉(zhuǎn)膜的效果也可以用麗春紅染色液對膜進行染色,以觀察實際的轉(zhuǎn)膜效果。也可以用考馬斯亮藍快速染色液對完成轉(zhuǎn)膜的SDS-PAGE膠進行染色,以觀察蛋白的殘留情況。
產(chǎn)品名稱:Anti-Megalin抗體,糖蛋白gp330抗體
產(chǎn)品編號:BYK-3909R
英文名稱:Anti-Megalin
中文名稱:糖蛋白gp330抗體
英文別名:Calcium sensor protein;
DBS;
Glycoprotein 330;
gp330;
Heymann nephritis antigen homolog;
Low-density lipoprotein receptor-related protein 2;
LRP-2;
Lrp2;
LRP2_HUMAN.
產(chǎn)品規(guī)格:0.1ml/0.2ml
此抗體本公司有單抗和多抗,可用于免疫組化(IHC),免疫印跡(WB),酶聯(lián)免疫(ELISA),IF、IP、流式細胞術(shù)等實驗(可來電索取說明)
保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
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