Anti-Alpha Dystroglycan抗體,肌相關(guān)蛋白DAG1抗體
英文介紹:Dystroglycan is a laminin binding component of the dystrophin-glycoprotein complex which provides a linkage between the subsarcolemmal cytoskeleton and the extracellular matrix. Dystroglycan 1 is a candidate gene for the site of the mutation in autosomal recessive muscular dystrophies. The dramatic reduction of dystroglycan 1 in Duchenne muscular dystrophy leads to a loss of linkage between the sarcolemma and extracellular matrix, rendering muscle fibers more susceptible to necrosis. Dystroglycan also functions as dual receptor for agrin and laminin-2 in the Schwann cell membrane. The muscle and nonmuscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence. Alternative splicing results in multiple transcript variants all encoding the same protein.
此產(chǎn)品本公司有標記的一抗出售,相關(guān)標記有:Alexa Fluor 350 標記、Alexa Fluor 488 標記、Alexa Fluor 555 標記、Alexa Fluor 647 標記、AP標記、APC標記、Biotin標記、Cy3標記、Cy5標記、Cy5.5標記、Cy7標記、FITC標記、Gold標記、HRP標記、PE標記、PE-Cy3標記、PE-CY5標記、PE-CY5.5標記、PE-CY7標記、RBITC標記
注意事項:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品名稱:Anti-Alpha Dystroglycan抗體,肌相關(guān)蛋白DAG1抗體
產(chǎn)品編號:BYK-5152R
英文名稱:Anti-Alpha Dystroglycan
中文名稱:肌相關(guān)蛋白DAG1抗體
英文別名:AGRNR; Alpha-DG; Beta-DG; Beta-dystroglycan; beta Dystroglycan; DAG; Dag1; DAG1_HUMAN; Dystroglycan 1 (dystrophin-associated glycoprotein 1); Dystroglycan; Dystrophin-associated glycoprotein 1; 156DAG; A3a; Dystrophin-associated glycoprotein 1.
產(chǎn)品規(guī)格:0.1ml/0.2ml
此抗體本公司有單抗和多抗,可用于免疫組化(IHC),免疫印跡(WB),酶聯(lián)免疫(ELISA),IF、IP、流式細胞術(shù)等實驗(可來電索取說明)
保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
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Anti-Alpha Dystroglycan抗體,肌相關(guān)蛋白DAG1抗體封閉(Blocking):
轉(zhuǎn)膜完畢后,立即把蛋白膜放置到預(yù)先準備好的Western洗滌液中,漂洗1-2分鐘,以洗去膜上的轉(zhuǎn)膜液。從轉(zhuǎn)膜完畢后所有的步驟,一定要注意膜的保濕,避免膜的干燥,否則極易產(chǎn)生較高的背景。
用微型臺式真空泵吸盡洗滌液,加入Western封閉液,在搖床上緩慢搖動,室溫封閉60分鐘。對于一些背景較高的抗體,可以在4℃ 封閉過夜。
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