產品名稱：Anti-PAP2B抗體,磷脂酸磷酸酶2b抗體

產品編號：BYK-4127R
英文名稱：Anti-PAP2B
中文名稱：磷脂酸磷酸酶2b抗體
英文別名：Dri42; Lipid phosphate phosphohydrolase 3; LPP3; PAP 2b; PAP2 beta; PAP2b; Vascular endothelial growth factor and type I collagen inducible protein; VCIP.
產品規格：0.1ml/0.2ml
此抗體本公司有單抗和多抗，可用于免疫組化（IHC），免疫印跡（WB），酶聯免疫(ELISA)，IF、IP、流式細胞術等實驗（可來電索取說明）
保存條件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Anti-PAP2B抗體,磷脂酸磷酸酶2b抗體

英文介紹：Phosphatidic acid phosphatase type 2b (PPAP2b) is a member of the phosphatidic acid phosphatase (PAP) family that function in de novo synthesis of glycerolipids as well as in receptor activated signal transduction mediated by phospholipase D. PAP2b catalyzes the conversion of phosphatidic acid to diacylglycerol and hydrolyzes lysophosphatidic acid, ceramide-1-phosphate and sphingosine-1-phosphate. It is a homodimer glycoprotein localized at the cell plasma membrane. Expression promotes adhesion, spreading, and tyrosine phosphorylation of Fak, Shc, Cas and paxillin in endothelial cells. PAP2b induced cell-cell interactions regulate specific intracellular signaling pathways that involve p120-catenin, Fak, Akt, and GSK3? protein kinases.
此產品本公司有標記的一抗出售，相關標記有：Alexa Fluor 350 標記、Alexa Fluor 488 標記、Alexa Fluor 555 標記、Alexa Fluor 647 標記、AP標記、APC標記、Biotin標記、Cy3標記、Cy5標記、Cy5.5標記、Cy7標記、FITC標記、Gold標記、HRP標記、PE標記、PE-Cy3標記、PE-CY5標記、PE-CY5.5標記、PE-CY7標記、RBITC標記
注意事項:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

Anti-PAP2B抗體,磷脂酸磷酸酶2b抗體封閉(Blocking):

轉膜完畢后，立即把蛋白膜放置到預先準備好的Western洗滌液中，漂洗1-2分鐘，以洗去膜上的轉膜液。從轉膜完畢后所有的步驟，一定要注意膜的保濕，避免膜的干燥，否則極易產生較高的背景。
用微型臺式真空泵吸盡洗滌液，加入Western封閉液，在搖床上緩慢搖動,室溫封閉60分鐘。對于一些背景較高的抗體，可以在4℃ 封閉過夜。

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