產品名稱:Anti-HLA Class 1 ABC/HLA ABC抗體,人類白細胞抗原A抗體
產品編號:BYK-2355R
英文名稱:Anti-HLA Class 1 ABC/HLA ABC
中文名稱:人類白細胞抗原A抗體
英文別名:HLA A; HLA B; HLA C; HLAabc; HLA class 1 A; HLA class 1 B; HLA class 1 C; Major histocompatibility complex, class I, A + B + C; MHC class I HLA A; MHC class I HLA B; MHC class I HLA C; MHC HLA ABC.
產品規格:0.1ml/0.2ml
此抗體本公司有單抗和多抗,可用于免疫組化(IHC),免疫印跡(WB),酶聯免疫(ELISA),IF、IP、流式細胞術等實驗(可來電索取說明)
保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Anti-HLA Class 1 ABC/HLA ABC抗體,人類白細胞抗原A抗體
英文介紹:Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin. Inhibited by the covalent attachment of herpes simplex virus ICP47 protein, which blocks the peptide-binding site of TAP. Inhibited by human cytomegalovirus US6 glycoprotein, which binds to the lumenal side of the TAP complex and inhibits peptide translocation by specifically blocking ATP-binding to TAP1 and prevents the conformational rearrangement of TAP induced by peptide binding. Inhibited by human adenovirus E3-19K glycoprotein, which binds the TAP complex and acts as a tapasin inhibitor, preventing MHC class I/TAP association. Expression of TAP1 is down-regulated by human Epstein-Barr virus vIL-10 protein, thereby affecting the transport of peptides into the endoplasmic reticulum and subsequent peptide loading by MHC class I molecules.
此產品本公司有標記的一抗出售,相關標記有:Alexa Fluor 350 標記、Alexa Fluor 488 標記、Alexa Fluor 555 標記、Alexa Fluor 647 標記、AP標記、APC標記、Biotin標記、Cy3標記、Cy5標記、Cy5.5標記、Cy7標記、FITC標記、Gold標記、HRP標記、PE標記、PE-Cy3標記、PE-CY5標記、PE-CY5.5標記、PE-CY7標記、RBITC標記
注意事項:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Anti-HLA Class 1 ABC/HLA ABC抗體,人類白細胞抗原A抗體抗體溶解方法及分裝:
1、購買抗體后,查看標簽上的規格,如:0.1ml、0.2ml
2、以規格為 0.1ml 的凍干粉抗體為例(部分抗體為液體,無需再溶解):
溶解方法一:用移液器加 0.1ml 雙蒸水到試管內,混勻,-20℃保存,若為其它規格(如:0.2ml)的凍干粉抗體,則加與標簽上規格*的(如:0.2ml)雙蒸水溶解,
混勻,-20℃保存。
溶解方法二:用移液器加 0.05ml(規格的 50%)雙蒸水到試管內,再加 0.05ml(規格的 50%)
的甘油到試管內,混勻,-20℃保存,若為其它規格(如:0.2ml)的凍干粉抗體,則加標簽上規格的 50%(如:0.1ml)雙蒸水,再加
標簽上規格的 50%(如:0.1ml)甘油溶解,混勻,-20℃保存。
抗體溶解后,將其分裝成 5-10 支(以 0.1ml 抗體為例,20ulx5 支或 10ulx10 支), -20℃保存,用
一支取一支,避免反復凍溶。
另:預試驗可做幾個梯度 1:100、1:200、1:400 稀釋,如果背景高,還可再稀釋,選一個zui
佳的稀釋比例,再正式做,效果。
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