產品名稱:Anti-TGF β1抗體,轉化生長因子β1/TGF-β1抗體
產品編號:BYK-0086R
英文名稱:Anti-TGF β1
中文名稱:轉化生長因子β1/TGF-β1抗體
英文別名:CED; DPD1; LAP; Latency-associated peptide; TGF beta 1; TGF beta; TGF beta 1 protein; TGF-beta 1 protein; TGF-beta-1; TGF-beta-5; TGF-beta1; TGFB; Tgfb-1; tgfb1; TGFB1_HUMAN; TGFbeta; TGFbeta1; Transforming Growth Factor b1; Transforming Growth Factor beta 1; Transforming growth factor beta 1a; transforming growth factor beta-1; transforming growth factor, beta 1.
產品規格:0.1ml/0.2ml
此抗體本公司有單抗和多抗,可用于免疫組化(IHC),免疫印跡(WB),酶聯免疫(ELISA),IF、IP、流式細胞術等實驗(可來電索取說明)
保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Anti-TGF β1抗體,轉化生長因子β1/TGF-β1抗體
英文介紹:This gene encodes a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types. Many cells have TGFB receptors, and the protein positively and negatively regulates many other growth factors. The secreted protein is cleaved into a latency-associated peptide (LAP) and a mature TGFB1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGFB1 homodimer. The mature peptide may also form composed of a TGFB1 homodimer. The mature peptide may also form composed of a TGFB1 homodimer. The mature peptide may also form composed of a TGFB1 homodimer. The mature peptide may also form composed of a TGFB1 homodimer. The mature peptide may also form heterodimers with other TGFB family members. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease.
此產品本公司有標記的一抗出售,相關標記有:Alexa Fluor 350 標記、Alexa Fluor 488 標記、Alexa Fluor 555 標記、Alexa Fluor 647 標記、AP標記、APC標記、Biotin標記、Cy3標記、Cy5標記、Cy5.5標記、Cy7標記、FITC標記、Gold標記、HRP標記、PE標記、PE-Cy3標記、PE-CY5標記、PE-CY5.5標記、PE-CY7標記、RBITC標記
注意事項:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Anti-TGF β1抗體,轉化生長因子β1/TGF-β1抗體上樣與電泳:
冷卻到室溫后,把蛋白樣品直接上樣到SDS-PAGE膠加樣孔內即可。為了便于觀察電泳效果和轉膜效果,以及判斷蛋白分子量大小,使用預染蛋白質分子量標準。
電泳時通常*在上層膠時使用低電壓恒壓電泳,而在溴酚藍進入下層膠時使用高電壓恒壓電泳。對于Bio-Rad的標準電泳裝置或類似電泳裝置,低電壓可以設置在80-100V,高電壓可以設置在120V左右。SDS-PAGE可以采用普通的電泳儀就可以滿足要求。為了電泳方便起見,也可以采用整個SDS-PAGE過程恒壓的方式,通常把電壓設置在100V,然后設定定時時間為90-120分鐘。設置定時可以避免經常發生的電泳過頭。
通常電泳時溴酚藍到達膠的底端處附近即可停止電泳,或者可以根據預染蛋白質分子量標準的電泳情況,預計目的蛋白已經被適當分離后即可停止電泳。
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