Anti-ERGI3抗體,內質網定位蛋白ERGI3抗體孵育(Primary antibody incubation)：

一抗孵育(Primary antibody incubation)
參考一抗的說明書，按照適當比例用Western一抗稀釋液稀釋一抗。
用微型臺式真空泵吸盡封閉液，立即加入稀釋好的一抗，室溫或4℃在側擺搖床上緩慢搖動孵育一小時。如果一抗孵育一小時效果不佳，可以在4℃緩慢搖動孵育過夜。回收一抗。加入Western洗滌液，在側擺搖床上緩慢搖動洗滌5-10分鐘。吸盡洗滌液后，再加入洗滌液，洗滌5-10分鐘。共洗滌3次。如果結果背景較高可以適當延長洗滌時間并增加洗滌次數。
二抗孵育(Secondary antibody inucubation)
參考二抗的說明書，按照適當比例用Western二抗稀釋液稀釋辣根過氧化物酶(HRP)標記的二抗。
用微型臺式真空泵吸盡洗滌液，立即加入稀釋好的二抗，室溫或4℃在側擺搖床上緩慢搖動孵育一小時。回收二抗，加入Western洗滌液，在側擺搖床上緩慢搖動洗滌5-10分鐘。吸盡洗滌液后，再加入洗滌液，洗滌5-10分鐘。共洗滌3次。如果結果背景較高可以適當延長洗滌時間并增加洗滌次數。

產品名稱:Anti-ERGI3抗體,內質網定位蛋白ERGI3抗體

產品編號：BYK-13103R
英文名稱：Anti-ERGI3
中文名稱：內質網定位蛋白ERGI3抗體
英文別名：2310015B14Rik; AV318804; C20orf47; CGI 54; D2Ucla1; dJ477O4.2; DKFZp547A2190; Endoplasmic reticulum Golgi intermediate compartment protein 3; endoplasmic reticulum localized protein ERp43; Endoplasmic reticulum-Golgi intermediate compartment protein 3; ERGI3_HUMAN; ERGIC and golgi 3; ergic3; ERV46; NY BR 84; PRO0989; RP23-220D12.2; RP3-477O4.1; SDBCAG84; serologically defined breast cancer antigen 84; Serologically defined breast cancer antigen NY BR 84; Serologically defined breast cancer antigen NY-BR-84.
產品規格：0.1ml/0.2ml
此抗體本公司有單抗和多抗，可用于免疫組化（IHC），免疫印跡（WB），酶聯免疫(ELISA)，IF、IP、流式細胞術等實驗（可來電索取說明）
保存條件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Anti-ERGI3抗體,內質網定位蛋白ERGI3抗體

英文介紹：Cycling proteins play important roles in the organization and function of the early secretory pathway by participating in membrane traffic and selective transport of cargo between the endoplasmic reticulum (ER), the intermediate compartment (ERGIC), and the Golgi. A family of membrane bound, ubiquitous proteins involved in the selective transport of newly synthesized glycoproteins from the ER to the ERGIC include VIP36, ERGIC-53, ERGIC-1, ERGIC-2 and ERGIC-3. ERGIC-1, also designated ERGIC32, is thought to modulate the activity of a complex formed by ERGIC-2, also designated Erv41, and ERGIC-3, also designated Erv46. ERGIC-2 and ERGIC-3 are both mammalian homologs of yeast proteins abundant in COPII-coated vesicles and localize to the Cis-face of the Golgi apparatus.
此產品本公司有標記的一抗出售，相關標記有：Alexa Fluor 350 標記、Alexa Fluor 488 標記、Alexa Fluor 555 標記、Alexa Fluor 647 標記、AP標記、APC標記、Biotin標記、Cy3標記、Cy5標記、Cy5.5標記、Cy7標記、FITC標記、Gold標記、HRP標記、PE標記、PE-Cy3標記、PE-CY5標記、PE-CY5.5標記、PE-CY7標記、RBITC標記
注意事項:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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