Anti-DRIP130抗體,轉錄共激活因子130抗體

英文介紹：The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein also acts as a metastasis suppressor. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene.
此產品本公司有標記的一抗出售，相關標記有：Alexa Fluor 350 標記、Alexa Fluor 488 標記、Alexa Fluor 555 標記、Alexa Fluor 647 標記、AP標記、APC標記、Biotin標記、Cy3標記、Cy5標記、Cy5.5標記、Cy7標記、FITC標記、Gold標記、HRP標記、PE標記、PE-Cy3標記、PE-CY5標記、PE-CY5.5標記、PE-CY7標記、RBITC標記
注意事項:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

產品名稱:Anti-DRIP130抗體,轉錄共激活因子130抗體

產品編號：BYK-13010R
英文名稱：Anti-DMC1
產品編號：BYK-13032R
英文名稱：Anti-DRIP130
中文名稱：轉錄共激活因子130抗體
英文別名：130 kDa transcriptional co activator; 133 kDa transcriptional co activator; Activator recruited cofactor 130 kDa component; Activator-recruited cofactor 130 kDa component; ARC 130; ARC130; Cofactor required for Sp1 transcriptional activation subunit 3 130kDa; Cofactor required for Sp1 transcriptional activation subunit 3 130kDa mediator; Cofactor required for Sp1 transcriptional activation subunit 3; CRSP 130; CRSP 130 kD subunit; CRSP 133; CRSP 3; CRSP complex subunit 3; CRSP130; CRSP133; CRSP3; CRSP3 protein; DKFZp434H0117; DRIP 130; DRIP130; hSur-2; KIAA1216; MED 23; MED23; MED23_HUMAN; Mediator complex subunit 23; Mediator of RNA polymerase II transcription subunit 23; Protein sur-2 homolog; SUR 2; SUR2; Transcriptional co activator CRSP 130; Transcriptional co activator CRSP130; Transcriptional coactivator CRSP130; Vitamin D3 receptor interacting protein; Vitamin D3 receptor interacting protein complex 130 kDa component; Vitamin D3 receptor-interacting protein complex 130 kDa component.
產品規(guī)格：0.1ml/0.2ml
此抗體本公司有單抗和多抗，可用于免疫組化（IHC），免疫印跡（WB），酶聯(lián)免疫(ELISA)，IF、IP、流式細胞術等實驗（可來電索取說明）
保存條件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Anti-DRIP130抗體,轉錄共激活因子130抗體Western Blotting 具體實驗步驟：

Western，也稱Western blot、Western blotting、Western 印跡，是用抗體檢測蛋白的重要方法之一。
Western可以參考如下步驟進行操作：
收集蛋白樣品(Protein sample preparation)
可以使用適當?shù)牧呀庖毫呀赓N壁細胞、懸浮細胞或組織樣品。對于某些特定的亞細胞組份蛋白，例如細胞核蛋白、細胞漿蛋白、線粒體蛋白等，可以參考相關文獻提取這些亞細胞組份蛋白，也可以使用試劑盒進行抽提。
收集完蛋白樣品后，為確保每個蛋白樣品的上樣量*，需要測定每個蛋白樣品的蛋白濃度。根據(jù)所使用的裂解液的不同，需要采用適當?shù)牡鞍诐舛葴y定方法。因為不同的蛋白濃度測定方法對于一些去垢劑和還原劑等的兼容性差別很大。

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