產(chǎn)品名稱:Anti-C1s抗體,補(bǔ)體C1S鏈多肽抗體
產(chǎn)品編號(hào):BYK-15088R
英文名稱:Anti-C1s
中文名稱:補(bǔ)體C1S鏈多肽抗體
英文別名:Basic proline rich peptide IB 1; C1 esterase; C1S; C1S_HUMAN; Complement C1s subcomponent; Complement C1s subcomponent heavy chain; Complement C1s subcomponent light chain; Complement component 1 s subcomponent; Complement component 1 subcomponent s; FLJ44757.
產(chǎn)品規(guī)格:0.1ml/0.2ml
此抗體本公司有單抗和多抗,可用于免疫組化(IHC),免疫印跡(WB),酶聯(lián)免疫(ELISA),IF、IP、流式細(xì)胞術(shù)等實(shí)驗(yàn)(可來電索取說明)
保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Anti-C1s抗體,補(bǔ)體C1S鏈多肽抗體Western Blotting 具體實(shí)驗(yàn)步驟:
Western,也稱Western blot、Western blotting、Western 印跡,是用抗體檢測(cè)蛋白的重要方法之一。
Western可以參考如下步驟進(jìn)行操作:
收集蛋白樣品(Protein sample preparation)
可以使用適當(dāng)?shù)牧呀庖毫呀赓N壁細(xì)胞、懸浮細(xì)胞或組織樣品。對(duì)于某些特定的亞細(xì)胞組份蛋白,例如細(xì)胞核蛋白、細(xì)胞漿蛋白、線粒體蛋白等,可以參考相關(guān)文獻(xiàn)提取這些亞細(xì)胞組份蛋白,也可以使用試劑盒進(jìn)行抽提。
收集完蛋白樣品后,為確保每個(gè)蛋白樣品的上樣量*,需要測(cè)定每個(gè)蛋白樣品的蛋白濃度。根據(jù)所使用的裂解液的不同,需要采用適當(dāng)?shù)牡鞍诐舛葴y(cè)定方法。因?yàn)椴煌牡鞍诐舛葴y(cè)定方法對(duì)于一些去垢劑和還原劑等的兼容性差別很大。
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Anti-C1s抗體,補(bǔ)體C1S鏈多肽抗體
英文介紹:The complement component proteins, C1, C3, C4, and C5, are potent anaphylatoxins that are released during complement activation. Binding of these proteins to their respective G protein-coupled receptors induces proinflammatory events, such as cellular degranulation, smooth muscle contraction, arachidonic acid metabolism, cytokine release, leukocyte activation, and cellular chemotaxis. C1q, together with proenzymes C1r and C1s, yield C1, the first component of the classical pathway of the serum complement system. C1 consists of a calcium dependent trimolecular complex of C1r, C1s and C1q in a 2:2:1 ratio. Activated C1s is in the form of a disulfide-linked heterodimer consisting of a heavy chain and a light chain. Defects in the gene encoding for C1s can cause selective C1s deficiency, a disorder characterized by early onset of various autoimmune diseases.
此產(chǎn)品本公司有標(biāo)記的一抗出售,相關(guān)標(biāo)記有:Alexa Fluor 350 標(biāo)記、Alexa Fluor 488 標(biāo)記、Alexa Fluor 555 標(biāo)記、Alexa Fluor 647 標(biāo)記、AP標(biāo)記、APC標(biāo)記、Biotin標(biāo)記、Cy3標(biāo)記、Cy5標(biāo)記、Cy5.5標(biāo)記、Cy7標(biāo)記、FITC標(biāo)記、Gold標(biāo)記、HRP標(biāo)記、PE標(biāo)記、PE-Cy3標(biāo)記、PE-CY5標(biāo)記、PE-CY5.5標(biāo)記、PE-CY7標(biāo)記、RBITC標(biāo)記
注意事項(xiàng):This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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