Anti-Exonuclease 1抗體,核酸外切酶1抗體產品現貨銷售，其他同類產品：

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產品名稱：Anti-Exonuclease 1抗體,核酸外切酶1抗體

產品編號：BYK-13119R
英文名稱：Anti-Exonuclease 1
中文名稱：核酸外切酶1抗體
英文別名：exo1; EXO1_HUMAN; ExoI; Exonuclease 1; Exonuclease I; Exonuclease1; HEX1; hExo I; hExo1; hExoI; Rad2 nuclease family member homolog of S. cerevisiae exonuclease 1.
產品規格：0.1ml/0.2ml
此抗體本公司有單抗和多抗，可用于免疫組化（IHC），免疫印跡（WB），酶聯免疫(ELISA)，IF、IP、流式細胞術等實驗（可來電索取說明）
保存條件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Anti-Exonuclease 1抗體,核酸外切酶1抗體Western Blotting 具體實驗步驟:

Western，也稱Western blot、Western blotting、Western 印跡，是用抗體檢測蛋白的重要方法之一。
Western可以參考如下步驟進行操作：
收集蛋白樣品(Protein sample preparation)
可以使用適當的裂解液裂解貼壁細胞、懸浮細胞或組織樣品。對于某些特定的亞細胞組份蛋白，例如細胞核蛋白、細胞漿蛋白、線粒體蛋白等，可以參考相關文獻提取這些亞細胞組份蛋白，也可以使用試劑盒進行抽提。
收集完蛋白樣品后，為確保每個蛋白樣品的上樣量*，需要測定每個蛋白樣品的蛋白濃度。根據所使用的裂解液的不同，需要采用適當的蛋白濃度測定方法。因為不同的蛋白濃度測定方法對于一些去垢劑和還原劑等的兼容性差別很大。

Anti-Exonuclease 1抗體,核酸外切酶1抗體

英文介紹：Comparative evaluation of the expression patterns of the human and mouse genes, combined with previous biochemical and yeast genetic studies, indicate that the Exo1 (Exonuclease I) proteins are important contributors to chromosome processing during mammalian DNA repair and recombination. In mice, the Exo1 gene maps to distal chromosome 1, consistent with the recent mapping of the orthologous human HEX1/EXO1 gene to chromosome 1q43. Exo1 is expressed prominently in testis, an area of active homologous recombination, and spleen, a prominent lymphoid tissue. In both mammalian and yeast systems, Exo1 is a 5'-3' double stranded DNA exonuclease that has previously been implicated in DNA mismatch repair (MMR). The MMR system ensures genome integrity by removing mispaired and unpaired bases that originate during replication. In humans, Exo1 interacts with MSH2 and MLH1 and has been proposed to be a redundant exonuclease in MMR. In both mammalian and yeast systems, Exo1 plays a structural role in MMR and stabilizes multiprotein complexes containing a number of MMR proteins.
此產品本公司有標記的一抗出售，相關標記有：Alexa Fluor 350 標記、Alexa Fluor 488 標記、Alexa Fluor 555 標記、Alexa Fluor 647 標記、AP標記、APC標記、Biotin標記、Cy3標記、Cy5標記、Cy5.5標記、Cy7標記、FITC標記、Gold標記、HRP標記、PE標記、PE-Cy3標記、PE-CY5標記、PE-CY5.5標記、PE-CY7標記、RBITC標記
注意事項:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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