產品名稱:Anti-CMKLR1抗體,趨化樣因子受體1抗體說明書
產品編號:BYK-10185R
英文名稱:Anti-CMKLR1
中文名稱:趨化樣因子受體1抗體
英文別名:Chemokine receptor-like 1; CHEMR23; ChemR23; DEZ; Chemokine like receptor 1; G protein coupled receptor ChemR23; G protein coupled receptor DEZ; CML1_HUMAN ; Orphan G protein coupled receptor.
產品規格:0.1ml/0.2ml
此抗體本公司有單抗和多抗,可用于免疫組化(IHC),免疫印跡(WB),酶聯免疫(ELISA),IF、IP、流式細胞術等實驗(可來電索取說明)
保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Anti-CMKLR1抗體,趨化樣因子受體1抗體說明書孵育(Primary antibody incubation):
一抗孵育(Primary antibody incubation)
參考一抗的說明書,按照適當比例用Western一抗稀釋液稀釋一抗。
用微型臺式真空泵吸盡封閉液,立即加入稀釋好的一抗,室溫或4℃在側擺搖床上緩慢搖動孵育一小時。如果一抗孵育一小時效果不佳,可以在4℃緩慢搖動孵育過夜。回收一抗。加入Western洗滌液,在側擺搖床上緩慢搖動洗滌5-10分鐘。吸盡洗滌液后,再加入洗滌液,洗滌5-10分鐘。共洗滌3次。如果結果背景較高可以適當延長洗滌時間并增加洗滌次數。
二抗孵育(Secondary antibody inucubation)
參考二抗的說明書,按照適當比例用Western二抗稀釋液稀釋辣根過氧化物酶(HRP)標記的二抗。
用微型臺式真空泵吸盡洗滌液,立即加入稀釋好的二抗,室溫或4℃在側擺搖床上緩慢搖動孵育一小時。回收二抗,加入Western洗滌液,在側擺搖床上緩慢搖動洗滌5-10分鐘。吸盡洗滌液后,再加入洗滌液,洗滌5-10分鐘。共洗滌3次。如果結果背景較高可以適當延長洗滌時間并增加洗滌次數。
Anti-CMKLR1抗體,趨化樣因子受體1抗體說明書
英文介紹:Chemokine-Like Receptor 1 (CMKLR1) is a serpentine transmembrane G-protein linked receptor. It is an orphan chemoattractant receptor, also known as ChemR23 or DEZ. CMKLR1 is expressed on the surface of monocyte-derived immature dendritic cells and peripheral plamacytoid dendritic (pDC) cells; but not on myeloid dendritic (mDC) cells, monocytes and lymphocytes. The expression of CMKLR1 on pDC is down-regulated after activation. Chemerin (TIG2) is a natural ligand of CMKLR1. The interaction of CMKLR1 with Chemerin induces pDC activation and chemotaxis. It has been shown that CMKLR1 is a coreceptor for several strains of SIV and HIV-1. CMKLR1 is a potential useful marker for distinguishing pDCs and mDCs.
此產品本公司有標記的一抗出售,相關標記有:Alexa Fluor 350 標記、Alexa Fluor 488 標記、Alexa Fluor 555 標記、Alexa Fluor 647 標記、AP標記、APC標記、Biotin標記、Cy3標記、Cy5標記、Cy5.5標記、Cy7標記、FITC標記、Gold標記、HRP標記、PE標記、PE-Cy3標記、PE-CY5標記、PE-CY5.5標記、PE-CY7標記、RBITC標記
注意事項:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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