產(chǎn)品名稱：Anti-CD35抗體,Ⅰ型補體受體抗體

產(chǎn)品編號：BYK-10144R
英文名稱：Anti-CD35
中文名稱：Ⅰ型補體受體抗體
英文別名：C3 binding protein; C3b/C4b receptor; C3BR; C4BR; CD 35; CD35; CD35 antigen; complement component (3b/4b) receptor 1 (Knops blood group); complement component (3b/4b) receptor 1 including Knops blood group system; Complement component receptor 1; Complement receptor 1; Complement receptor type 1; CR 1; CR1; CR1_HUMAN; KN; Knops blood group antigen.
產(chǎn)品規(guī)格：0.1ml/0.2ml
此抗體本公司有單抗和多抗，可用于免疫組化（IHC），免疫印跡（WB），酶聯(lián)免疫(ELISA)，IF、IP、流式細胞術(shù)等實驗（可來電索取說明）
保存條件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Anti-CD35抗體,Ⅰ型補體受體抗體產(chǎn)品現(xiàn)貨銷售，其他同類產(chǎn)品：

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Anti-CD35抗體,Ⅰ型補體受體抗體

英文介紹：This gene is a member of the receptors of complement activation (RCA) family and is located in the 'cluster RCA' region of chromosome 1. The gene encodes a monomeric single-pass type I membrane glycoprotein found on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells. The Knops blood group system is a system of antigens located on this protein. The protein mediates cellular binding to particles and immune complexes that have activated complement. Decreases in expression of this protein and/or mutations in its gene have been associated with gallbladder carcinomas, mesangiocapillary glomerulonephritis, systemic lupus erythematosus and sarcoidosis. Mutations in this gene have also been associated with a reduction in Plasmodium falciparum rosetting, conferring protection against severe malaria. Alternate allele-specific splice variants, encoding different isoforms, have been characterized. Additional allele specific isoforms, including a secreted form, have been described but have not been fully characterized.
此產(chǎn)品本公司有標(biāo)記的一抗出售，相關(guān)標(biāo)記有：Alexa Fluor 350 標(biāo)記、Alexa Fluor 488 標(biāo)記、Alexa Fluor 555 標(biāo)記、Alexa Fluor 647 標(biāo)記、AP標(biāo)記、APC標(biāo)記、Biotin標(biāo)記、Cy3標(biāo)記、Cy5標(biāo)記、Cy5.5標(biāo)記、Cy7標(biāo)記、FITC標(biāo)記、Gold標(biāo)記、HRP標(biāo)記、PE標(biāo)記、PE-Cy3標(biāo)記、PE-CY5標(biāo)記、PE-CY5.5標(biāo)記、PE-CY7標(biāo)記、RBITC標(biāo)記
注意事項:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

Anti-CD35抗體,Ⅰ型補體受體抗體轉(zhuǎn)膜(Transfer):

我們*在Western實驗中選用PVDF膜。硝酸纖維素膜(NC膜)或PVDF膜（二氟化樹脂膜膜孔徑：0.45um)也可以使用，但硝酸纖維素膜比較脆，在操作過程中特別是用鑷子夾取等過程中容易裂開。膜的使用請參考生產(chǎn)商的*使用步驟。
通常如果使用Bio-Rad的標(biāo)準濕式轉(zhuǎn)膜裝置，可以設(shè)定轉(zhuǎn)膜電壓120V，轉(zhuǎn)膜時間為30-60分鐘。具體的轉(zhuǎn)膜時間要根據(jù)目的蛋白的大小而定，目的蛋白的分子量越大，需要的轉(zhuǎn)膜時間越長，目的蛋白的分子量越小，需要的轉(zhuǎn)膜時間越短。
在轉(zhuǎn)膜過程中，特別是高電流快速轉(zhuǎn)膜時，通常會有非常嚴重的發(fā)熱現(xiàn)象，把轉(zhuǎn)膜槽放置在冰浴中進行轉(zhuǎn)膜。轉(zhuǎn)膜的效果可以觀察所使用的預(yù)染蛋白質(zhì)分子量標(biāo)準，通常分子量zui大的1-2條帶較難全部轉(zhuǎn)到膜上。轉(zhuǎn)膜的效果也可以用麗春紅染色液對膜進行染色，以觀察實際的轉(zhuǎn)膜效果。也可以用考馬斯亮藍快速染色液對完成轉(zhuǎn)膜的SDS-PAGE膠進行染色，以觀察蛋白的殘留情況。

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