Anti-DAZL抗體,生細胞發育相關蛋白DAZL抗體抗體溶解方法及分裝:
1、購買抗體后,查看標簽上的規格,如:0.1ml、0.2ml
2、以規格為 0.1ml 的凍干粉抗體為例(部分抗體為液體,無需再溶解):
溶解方法一:用移液器加 0.1ml 雙蒸水到試管內,混勻,-20℃保存,若為其它規格(如:0.2ml)的凍干粉抗體,則加與標簽上規格*的(如:0.2ml)雙蒸水溶解,
混勻,-20℃保存。
溶解方法二:用移液器加 0.05ml(規格的 50%)雙蒸水到試管內,再加 0.05ml(規格的 50%)
的甘油到試管內,混勻,-20℃保存,若為其它規格(如:0.2ml)的凍干粉抗體,則加標簽上規格的 50%(如:0.1ml)雙蒸水,再加
標簽上規格的 50%(如:0.1ml)甘油溶解,混勻,-20℃保存。
抗體溶解后,將其分裝成 5-10 支(以 0.1ml 抗體為例,20ulx5 支或 10ulx10 支), -20℃保存,用
一支取一支,避免反復凍溶。
另:預試驗可做幾個梯度 1:100、1:200、1:400 稀釋,如果背景高,還可再稀釋,選一個zui
佳的稀釋比例,再正式做,效果。
產品名稱:Anti-DAZL抗體,生細胞發育相關蛋白DAZL抗體
產品編號:BYK-12245R
英文名稱:Anti-DAZL
中文名稱:生細胞發育相關蛋白DAZL抗體
英文別名:DAZ homolog; DAZ like autosomal; DAZ-like autosomal; deleted in azoospermia-like; DAZH; DAZL 1; DAZL; DAZL_HUMAN; DAZL1; DAZLA; Deleted in azoospermia like 1; Deleted in azoospermia like; Deleted in azoospermia like autosomal; Deleted in azoospermia-like 1; Deleted in azoospermia-like; Germline specific RNA binding protein; MGC26406; Spermatogenesis gene on the Y like autosomal; SPGY like autosomal; SPGY-like-autosomal; SPGYLA; Tpx2.
產品規格:0.1ml/0.2ml
此抗體本公司有單抗和多抗,可用于免疫組化(IHC),免疫印跡(WB),酶聯免疫(ELISA),IF、IP、流式細胞術等實驗(可來電索取說明)
保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Anti-DAZL抗體,生細胞發育相關蛋白DAZL抗體
英文介紹:The DAZ (Deleted in AZoospermia) gene family encodes potential RNA binding proteins that are expressed in prenatal and postnatal germ cells of males and females. The protein encoded by this gene is localized to the nucleus and cytoplasm of fetal germ cells and to the cytoplasm of developing oocytes. In the testis, this protein is localized to the nucleus of spermatogonia but relocates to the cytoplasm during meiosis where it persists in spermatids and spermatozoa. Transposition and amplification of this autosomal gene during primate evolution gave rise to the DAZ gene cluster on the Y chromosome. Mutations in this gene have been linked to severe spermatogenic failure and infertility in males. Two transcript variants encoding different isoforms have been found for this gene.
此產品本公司有標記的一抗出售,相關標記有:Alexa Fluor 350 標記、Alexa Fluor 488 標記、Alexa Fluor 555 標記、Alexa Fluor 647 標記、AP標記、APC標記、Biotin標記、Cy3標記、Cy5標記、Cy5.5標記、Cy7標記、FITC標記、Gold標記、HRP標記、PE標記、PE-Cy3標記、PE-CY5標記、PE-CY5.5標記、PE-CY7標記、RBITC標記
注意事項:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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