產(chǎn)品名稱：Anti-GPR 151抗體,G蛋白偶聯(lián)受體151抗體價(jià)格

產(chǎn)品編號(hào)：BYK-13505R
英文名稱：Anti-GPR 151
中文名稱：G蛋白偶聯(lián)受體151/GPCR 2037抗體
英文別名：G protein coupled receptor 151; G protein coupled receptor PGR7; G-protein coupled receptor PGR7; Galanin receptor 4; Galanin receptor like; GALR4; GALRL; GP151_HUMAN; GPCR; GPCR-2037; GPCR2037; GPR 151; GPR151; PGR7; Probable G protein coupled receptor 151; Probable G-protein coupled receptor 151; Putative G protein coupled receptor; GPCR GPR151.
產(chǎn)品規(guī)格：0.1ml/0.2ml
此抗體本公司有單抗和多抗，可用于免疫組化（IHC），免疫印跡（WB），酶聯(lián)免疫(ELISA)，IF、IP、流式細(xì)胞術(shù)等實(shí)驗(yàn)（可來(lái)電索取說(shuō)明）
保存條件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Anti-GPR 151抗體,G蛋白偶聯(lián)受體151抗體價(jià)格孵育(Primary antibody incubation)：

一抗孵育(Primary antibody incubation)
參考一抗的說(shuō)明書(shū)，按照適當(dāng)比例用Western一抗稀釋液稀釋一抗。
用微型臺(tái)式真空泵吸盡封閉液，立即加入稀釋好的一抗，室溫或4℃在側(cè)擺搖床上緩慢搖動(dòng)孵育一小時(shí)。如果一抗孵育一小時(shí)效果不佳，可以在4℃緩慢搖動(dòng)孵育過(guò)夜。回收一抗。加入Western洗滌液，在側(cè)擺搖床上緩慢搖動(dòng)洗滌5-10分鐘。吸盡洗滌液后，再加入洗滌液，洗滌5-10分鐘。共洗滌3次。如果結(jié)果背景較高可以適當(dāng)延長(zhǎng)洗滌時(shí)間并增加洗滌次數(shù)。
二抗孵育(Secondary antibody inucubation)
參考二抗的說(shuō)明書(shū)，按照適當(dāng)比例用Western二抗稀釋液稀釋辣根過(guò)氧化物酶(HRP)標(biāo)記的二抗。
用微型臺(tái)式真空泵吸盡洗滌液，立即加入稀釋好的二抗，室溫或4℃在側(cè)擺搖床上緩慢搖動(dòng)孵育一小時(shí)。回收二抗，加入Western洗滌液，在側(cè)擺搖床上緩慢搖動(dòng)洗滌5-10分鐘。吸盡洗滌液后，再加入洗滌液，洗滌5-10分鐘。共洗滌3次。如果結(jié)果背景較高可以適當(dāng)延長(zhǎng)洗滌時(shí)間并增加洗滌次數(shù)。

英文介紹：GPCR2037 is a G protein-coupled receptor that undergoes weak activation by Galanin and is most abundant in the central nervous system (CNS), where it appears to be critical for development. During embryonal development the expression of GPCR2037 is widespread in the nervous system (dorsal thalamus, striatum, locus coeruleus and hindbrain nuclei). GPCR2037 in the CNS of 7- and 15-day-old mouse embryos can localize to the habenular complex. Low levels of GPCR2037 are detectable in testis, liver, kidney and stomach. In addition to GPCR2037, Galanin mediates its effects through receptor subtypes GALR1, 2 and 3. Galanin ligand exerts anxiolytic actions via GALR receptors under conditions of high stress. Galanin coexists with norepinephrine and serotonin in neural systems that mediate emotion.
此產(chǎn)品本公司有標(biāo)記的一抗出售，相關(guān)標(biāo)記有：Alexa Fluor 350 標(biāo)記、Alexa Fluor 488 標(biāo)記、Alexa Fluor 555 標(biāo)記、Alexa Fluor 647 標(biāo)記、AP標(biāo)記、APC標(biāo)記、Biotin標(biāo)記、Cy3標(biāo)記、Cy5標(biāo)記、Cy5.5標(biāo)記、Cy7標(biāo)記、FITC標(biāo)記、Gold標(biāo)記、HRP標(biāo)記、PE標(biāo)記、PE-Cy3標(biāo)記、PE-CY5標(biāo)記、PE-CY5.5標(biāo)記、PE-CY7標(biāo)記、RBITC標(biāo)記
注意事項(xiàng):This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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