Anti-HDGFRP3抗體,肝癌衍生生長因子相關蛋白3抗體

英文介紹：Hepatoma derived growth factor (HDGF) is the original member of a family of polypeptides designated HDGF-related proteins (HRPs). HDGF was initially characterized as a secreted mitogen from the Huh-7 human hepatoma cell line. This nuclear targeted vascular smooth muscle cell mitogen (VSM) is a heparin-binding protein that is highly expressed in tumor cells, where it stimulates proliferation. HDGF is also reported to be involved in organ development and lung remodeling after injury by promoting proliferation of lung epithelial cells. During development, HDGF expression is high in the nucleus and cytoplasm of smooth muscle and endothelial cells. The HRP family contains four proteins, HRP-1, HRP-2, HRP-3 and HRP-4. HRP-1 and HRP-4 are expressed only in testis, while HRP-2 is widely expressed in different tissues. HRP-3 can be found solely in the nervous system. Specifically it is strongly expressed in bulbus, olfactorius, piriform cotrex and amygdala complex, while HRP-2 in brain is located in the the thalamus, prefrontal and parietal cortex, neurohypophysis and cerebellum. In the central nervous system, HRPs play a role in neuron proliferation and cell survival.
此產品本公司有標記的一抗出售，相關標記有：Alexa Fluor 350 標記、Alexa Fluor 488 標記、Alexa Fluor 555 標記、Alexa Fluor 647 標記、AP標記、APC標記、Biotin標記、Cy3標記、Cy5標記、Cy5.5標記、Cy7標記、FITC標記、Gold標記、HRP標記、PE標記、PE-Cy3標記、PE-CY5標記、PE-CY5.5標記、PE-CY7標記、RBITC標記
注意事項:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

產品名稱：Anti-HDGFRP3抗體,肝癌衍生生長因子相關蛋白3抗體

產品編號：BYK-16464R
英文名稱：Anti-HDGFRP3
中文名稱：肝癌衍生生長因子相關蛋白3抗體
英文別名：CGI 142; 

HDGF 2; 

HDGF-2; 

HDGF2; 

HDGFRP 3; 

Hdgfrp3; 

HDGR3_HUMAN; 

Hepatoma derived growth factor 2; 

Hepatoma derived growth factor related protein 3; 

Hepatoma-derived growth factor 2; 

Hepatoma-derived growth factor-related protein 3; 

HRP 3; 

HRP-3; 

HRP3.
產品規格：0.1ml/0.2ml
此抗體本公司有單抗和多抗，可用于免疫組化（IHC），免疫印跡（WB），酶聯免疫(ELISA)，IF、IP、流式細胞術等實驗（可來電索取說明）
保存條件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Anti-HDGFRP3抗體,肝癌衍生生長因子相關蛋白3抗體Western Blotting 具體實驗步驟：

Western，也稱Western blot、Western blotting、Western 印跡，是用抗體檢測蛋白的重要方法之一。
Western可以參考如下步驟進行操作：

收集蛋白樣品(Protein sample preparation)
可以使用適當的裂解液裂解貼壁細胞、懸浮細胞或組織樣品。對于某些特定的亞細胞組份蛋白，例如細胞核蛋白、細胞漿蛋白、線粒體蛋白等，可以參考相關文獻提取這些亞細胞組份蛋白，也可以使用試劑盒進行抽提。
收集完蛋白樣品后，為確保每個蛋白樣品的上樣量*，需要測定每個蛋白樣品的蛋白濃度。根據所使用的裂解液的不同，需要采用適當的蛋白濃度測定方法。因為不同的蛋白濃度測定方法對于一些去垢劑和還原劑等的兼容性差別很大。

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