產品名稱: Anti-phospho-Zyxin (Ser142)抗體,磷酸化斑聯蛋白抗體實驗主打
產品編號:BYK-18547R
英文名稱:Anti-phospho-Zyxin (Ser142)
中文名稱:磷酸化斑聯蛋白抗體
英文別名:Zyxin (phospho S142); p-Zyxin (phospho S142); ESP 2; ESP2; HED 2; HED2; ZYX; ZYX protein; Zyxin 2; Zyxin2; ZYX_HUMAN.
英文介紹:Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform..
產品規格:0.1ml/0.2ml
注意事項:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
此抗體本公司有單抗和多抗,可用于免疫組化(IHC),免疫印跡(WB),酶聯免疫(ELISA),IF、IP、流式細胞術等實驗(可來電索取說明)
此產品本公司有標記的一抗出售,相關標記有:Alexa Fluor 350 標記、Alexa Fluor 488 標記、Alexa Fluor 555 標記、Alexa Fluor 647 標記、AP標記、APC標記、Biotin標記、Cy3標記、Cy5標記、Cy5.5標記、Cy7標記、FITC標記、Gold標記、HRP標記、PE標記、PE-Cy3標記、PE-CY5標記、PE-CY5.5標記、PE-CY7標記、RBITC標記
保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Anti-phospho-Zyxin (Ser142)抗體,磷酸化斑聯蛋白抗體實驗主打免疫組化染色:
SP法
(1) 脫蠟、水化 (2)PBS洗2-3次各5分鐘 (3)3%H2O2(80%甲醇)滴加在
TMA上,室溫靜置10分鐘 (4)PBS洗2-3次各5分鐘 (5)抗原修復
(6)PBS洗2-3次各5分鐘 (7)滴加正常山羊血清封閉液,室溫20分鐘,甩去多余液體(8)滴加Ι抗50μl,室溫靜置1小時或4℃過夜或37℃1小時(9)4℃過夜后需在37℃復溫45分鐘(10)PBS洗3次每次2分鐘
(11)滴加Ⅱ抗45-50μl,室溫靜置或37℃1小時(12)Ⅱ抗中可加入0.05%的
tween-20.(13)PBS洗3次各5分鐘(14)DAB顯色5-10分鐘,在顯微鏡下掌握染色程度 (15)PBS或自來水沖洗10分鐘 (16)蘇木精復染2分鐘,鹽酸酒精分化(17)自來水沖洗10-15分鐘 (18)脫水、透明、封片、鏡檢。
SABC法
(1) 脫蠟、水化 (2)PBS洗2次各5分鐘 (3)用蒸餾水或PBS配制新鮮的3%H2O2,室溫封閉5-10分鐘,用蒸餾水洗3次(4)抗原修復
(5) PBS洗5分鐘 (6)滴加正常山羊血清封閉液,室溫20分鐘,甩去多余液體(7)滴加Ι抗50μl,室溫靜置1小時或4℃過夜或37℃1小時
(8)PBS洗3次各2分鐘(9)滴加*化Ⅱ抗,20℃-37℃ 20分鐘
(10)PBS洗3次各2分鐘 (11)滴加試劑SABC 20℃-30℃ 20分鐘
(12) PBS洗4次各5分鐘 (13) DAB顯色:試劑盒或自配顯色劑顯色
(14)脫水、透明、封片、鏡檢。
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