產品名稱:Anti-LITAF抗體,脂多糖誘導腫瘤壞死因子α抗體技術說明
產品編號:BYK-18300R
英文名稱:Anti-LITAF
中文名稱:脂多糖誘導腫瘤壞死因子α抗體
英文別名:Lipopolysaccharide induced TNF alpha factor;
CMT1C;
FLJ38636;
Lipopolysaccharide induced TNF alpha factor;
Lipopolysaccharide induced TNF factor;
Lipopolysaccharide induced tumor necrosis factor alpha factor;
Lipopolysaccharide-induced tumor necrosis factor-alpha factor;
LITAF;
LITAF_HUMAN;
LPS induced TNF alpha factor;
LPS-induced TNF-alpha factor;
MGC116698;
MGC116700;
MGC116701;
MGC125274;
MGC125275;
MGC125276;
p53 induced gene 7 protein;
p53-induced gene 7 protein;
PIG 7;
PIG7;
SIMPLE;
Small integral membrane protein of lysosome/late endosome;
TP53I7;
Tumor protein p53 inducible protein 7.
英文介紹:Lipopolysaccharide is a potent stimulator of monocytes and macrophages, causing secretion of tumor necrosis factor-alpha (TNF-alpha) and other inflammatory mediators. This gene encodes lipopolysaccharide-induced TNF-alpha factor, which is a DNA-binding protein and can mediate the TNF-alpha expression by direct binding to the promoter region of the TNF-alpha gene. The transcription of this gene is induced by tumor suppresor p53 and has been implicated in the p53-induced apoptotic pathway. Mutations in this gene cause Charcot-Marie-Tooth disease type 1C (CMT1C) and may be involved in the carcinogenesis of extramammary Paget's disease (EMPD). Multiple alternatively spliced transcript variants have been found for this gene.
產品規格:0.1ml/0.2ml
注意事項:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
此抗體本公司有單抗和多抗,可用于免疫組化(IHC),免疫印跡(WB),酶聯免疫(ELISA),IF、IP、流式細胞術等實驗(可來電索取說明)
此產品本公司有標記的一抗出售,相關標記有:Alexa Fluor 350 標記、Alexa Fluor 488 標記、Alexa Fluor 555 標記、Alexa Fluor 647 標記、AP標記、APC標記、Biotin標記、Cy3標記、Cy5標記、Cy5.5標記、Cy7標記、FITC標記、Gold標記、HRP標記、PE標記、PE-Cy3標記、PE-CY5標記、PE-CY5.5標記、PE-CY7標記、RBITC標記
保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Anti-LITAF抗體,脂多糖誘導腫瘤壞死因子α抗體技術說明 免疫組化染色:
SP法
(1) 脫蠟、水化 (2)PBS洗2-3次各5分鐘 (3)3%H2O2(80%甲醇)滴加在
TMA上,室溫靜置10分鐘 (4)PBS洗2-3次各5分鐘 (5)抗原修復
(6)PBS洗2-3次各5分鐘 (7)滴加正常山羊血清封閉液,室溫20分鐘,甩去多余液體(8)滴加Ι抗50μl,室溫靜置1小時或4℃過夜或37℃1小時(9)4℃過夜后需在37℃復溫45分鐘(10)PBS洗3次每次2分鐘
(11)滴加Ⅱ抗45-50μl,室溫靜置或37℃1小時(12)Ⅱ抗中可加入0.05%的
tween-20.(13)PBS洗3次各5分鐘(14)DAB顯色5-10分鐘,在顯微鏡下掌握染色程度 (15)PBS或自來水沖洗10分鐘 (16)蘇木精復染2分鐘,鹽酸酒精分化(17)自來水沖洗10-15分鐘 (18)脫水、透明、封片、鏡檢。
SABC法
(1) 脫蠟、水化 (2)PBS洗2次各5分鐘 (3)用蒸餾水或PBS配制新鮮的3%H2O2,室溫封閉5-10分鐘,用蒸餾水洗3次(4)抗原修復
(5) PBS洗5分鐘 (6)滴加正常山羊血清封閉液,室溫20分鐘,甩去多余液體(7)滴加Ι抗50μl,室溫靜置1小時或4℃過夜或37℃1小時
(8)PBS洗3次各2分鐘(9)滴加*化Ⅱ抗,20℃-37℃ 20分鐘
(10)PBS洗3次各2分鐘 (11)滴加試劑SABC 20℃-30℃ 20分鐘
(12) PBS洗4次各5分鐘 (13) DAB顯色:試劑盒或自配顯色劑顯色
(14)脫水、透明、封片、鏡檢。
Anti-LITAF抗體,脂多糖誘導腫瘤壞死因子α抗體技術說明 其他優勢產品:
byk-0717R 精zi相關抗原5抗體 Anti-SPAG5/MAP126/Astrin
byk-11602R STAC1蛋白抗體 Anti-STAC
byk-1602R 信號感應增殖相關蛋白1抗體 Anti-SPA-1/SIPA-1
byk-0770R 肺表面活性蛋白A抗體 Anti-SP-A
byk-1545R 組織型纖溶酶原激活劑抗體 Anti-TPA/PLAT
byk-11545R B淋巴細胞白血病/淋巴瘤11B抗體 Anti-Ctip2
byk-0975R 轉錄生長因子SP1抗體 Anti-TSFP1/SP1
byk-0065R P物質抗體 Anti-Substance P
byk-10065R 軸突生長誘向因子4抗體 Anti-Netrin 4
byk-0523R 胚胎干細胞關鍵蛋白抗體 Anti-SOX2
byk-1132R *抗體 Anti-GRIH
byk-11132R 核纖層蛋白B2抗體 Anti-Lamin B2
byk-0473R 溶質攜帶物家族-1抗體 Anti-SLC1A5
byk-1080R 超氧化物歧化酶2抗體 Anti-SOD2
byk-11080R 髓鞘蛋白P0樣蛋白2抗體 Anti-MPZL2/EVA1
byk-1079R 超氧化物歧化酶1抗體(銅/鋅過氧化物歧化酶SOD) Anti-SOD1/SOD
byk-11079R 鈣粘附蛋白9抗體 Anti-Cadherin 9
byk-0580R 細胞因子信號傳導抑制蛋白3抗體 Anti-SOCS3
byk-1896R 細胞因子信號傳導抑制蛋白2抗體 Anti-Socs 2
byk-11896R 腦特異性跨膜蛋白抗體 Anti-NETO2
byk-0113R 細胞因子信號傳導抑制蛋白1抗體 Anti-SOCS1
byk-10113R 磷酸化微管相關蛋白抗體 Anti-phospho-Tau protein(Thr522)
byk-1371R Snail蛋白抗體 Anti-Snail/SNAI 1
byk-11371R 神經突觸素3抗體 Anti-Synapsin III
byk-1131R 突觸相關蛋白25抗體 Anti-SNAP25
byk-11131R 乳清蛋白α抗體 Anti-LALBA/alpha Lactalbumin
byk-0763R 蛇毒蛋白抗體(中華眼鏡蛇) Anti-Snake poison Protein
byk-0602R 蝮蛇蛇毒蛋白抗體 Anti-Echidnotoxin
byk-2665R 衰老標記蛋白30抗體 Anti-Regucalcin/SMP30
byk-2178R 細胞骨架相關蛋白抗體 Anti-SM22 Alpha
byk-12178R 鉀通道多聚體結構域KCTD18抗體 Anti-KCTD18
byk-1956R 平滑肌肌球蛋白重鏈抗體 Anti-SMMHC/Myosin
byk-11956R 神經突觸相關蛋白SLITRK3抗體 Anti-SLITRK3
咨詢及訂購的方法:
1.可來電向我們直接咨詢及訂購。:021-6034 0820,158 0061 4926
2.可加客服或交談,向我司在線人員咨詢及訂購。
3.可通過郵件咨詢及訂購。