產(chǎn)品名稱：抗體Anti-NF-H抗體,高分子量神經(jīng)絲蛋白抗體優(yōu)質(zhì)產(chǎn)品

產(chǎn)品編號：BYK-10680R
英文名稱：Anti-NF-H
中文名稱：高分子量神經(jīng)絲蛋白抗體
英文別名：Neurofilament 200; 200 kDa neurofilament protein; 200 kD Neurofilament Heavy; NEFH; NEFH; NF200; NF-200; Neurofilament H; Neurofilament heavy polypeptide 200kD; Neurofilament heavy polypeptide 200kDa; Neurofilament heavy polypeptide; Neurofilament triplet H protein; Neurofilament triplet H protein; Hypophosphorylated Neurofilament H; NF H; NF-H; NFH; NFH_HUMAN; KIAA0845.
英文介紹：Neurofilaments can be defined as the intermediate or 10nm filaments found in specifically in neuronal cells. When visualised using an electron microscope, neurofilaments appear as 10nm diameter fibres of indeterminate length that generally have fine wispy protrusions from their sides. They are particularly abundant in axons of large projection neurons. They probably function to provide structural support for neurons and their synapses and to support the large axon diameters required for rapid conduction of impulses down axons. Neurofilaments are composed of a mixture of subunits, which usually includes the three neurofilament triplet proteins neurofilament light (NFL), neurofilament medium (NFM) and neurofilament heavy (NFH). Neurofilaments may also include smaller amounts of peripherin, alpha internexin, nestin and in some cases vimentin. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons, and are biochemically very stable. Some studies have shown that levels of neurofilament heavy and neurofilament light are elevated in patients with Alzheimer's disease, frontotemporal lobe dementia, and vascular dementia.

產(chǎn)品規(guī)格：0.1ml/0.2ml

注意事項:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

此抗體本公司有單抗和多抗，可用于免疫組化（IHC），免疫印跡（WB），酶聯(lián)免疫(ELISA)，IF、IP、流式細胞術(shù)等實驗（可來電索取說明）
此產(chǎn)品本公司有標記的一抗出售，相關(guān)標記有：Alexa Fluor 350 標記、Alexa Fluor 488 標記、Alexa Fluor 555 標記、Alexa Fluor 647 標記、AP標記、APC標記、Biotin標記、Cy3標記、Cy5標記、Cy5.5標記、Cy7標記、FITC標記、Gold標記、HRP標記、PE標記、PE-Cy3標記、PE-CY5標記、PE-CY5.5標記、PE-CY7標記、RBITC標記

保存條件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

抗體Anti-NF-H抗體,高分子量神經(jīng)絲蛋白抗體優(yōu)質(zhì)產(chǎn)品免疫組化染色：
SP法
（1） 脫蠟、水化 （2）PBS洗2-3次各5分鐘 （3）3%H2O2（80%甲醇）滴加在
TMA上，室溫靜置10分鐘 （4）PBS洗2-3次各5分鐘 （5）抗原修復
（6）PBS洗2-3次各5分鐘 （7）滴加正常山羊血清封閉液，室溫20分鐘，甩去多余液體（8）滴加Ι抗50μl,室溫靜置1小時或4℃過夜或37℃1小時（9）4℃過夜后需在37℃復溫45分鐘（10）PBS洗3次每次2分鐘
（11）滴加Ⅱ抗45-50μl,室溫靜置或37℃1小時（12）Ⅱ抗中可加入0.05%的
tween-20.（13）PBS洗3次各5分鐘（14）DAB顯色5-10分鐘，在顯微鏡下掌握染色程度 （15）PBS或自來水沖洗10分鐘 （16）蘇木精復染2分鐘，鹽酸酒精分化（17）自來水沖洗10-15分鐘 （18）脫水、透明、封片、鏡檢。
SABC法
（1） 脫蠟、水化 （2）PBS洗2次各5分鐘 （3）用蒸餾水或PBS配制新鮮的3%H2O2，室溫封閉5-10分鐘，用蒸餾水洗3次（4）抗原修復
（5） PBS洗5分鐘 （6）滴加正常山羊血清封閉液，室溫20分鐘，甩去多余液體（7）滴加Ι抗50μl,室溫靜置1小時或4℃過夜或37℃1小時
（8）PBS洗3次各2分鐘（9）滴加*化Ⅱ抗，20℃-37℃ 20分鐘
（10）PBS洗3次各2分鐘 （11）滴加試劑SABC 20℃-30℃ 20分鐘
（12） PBS洗4次各5分鐘 （13） DAB顯色：試劑盒或自配顯色劑顯色
（14）脫水、透明、封片、鏡檢。

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