產(chǎn)品名稱：Anti-CNG3抗體,視錐感光細胞環(huán)磷酸鳥苷門控通道蛋白CNG3抗體技術(shù)說明

產(chǎn)品編號：BYK-10772R
英文名稱：Anti-CNG3
中文名稱：視錐感光細胞環(huán)磷酸鳥苷門控通道蛋白CNG3抗體
英文別名：ACHM2; CCNC1; CCNCa; CCNCalpha; CNCG3; CNG 3; CNG-3; CNG channel alpha 3; CNG channel alpha-3; CNG-3; CNG3; CNGA3; Cnga3 cyclic nucleotide gated channel alpha 3; CNGA3_HUMAN; Cone photoreceptor cGMP gated channel; Cone photoreceptor cGMP gated channel alpha subunit; Cone photoreceptor cGMP gated channel subunit alpha; Cone photoreceptor cGMP-gated channel subunit alpha; Cyclic nucleotide gated cation channel alpha 3; Cyclic nucleotide gated channel alpha 3; Cyclic nucleotide gated channel olfactory 3; Cyclic nucleotide gated channel rod photoreceptor subunit alpha; Cyclic nucleotide-gated cation channel alpha-3; Cyclic nucleotide-gated channel alpha-3.
英文介紹：Cyclic nucleotide-gated (CNG) cation channels are heteromeric complexes made up of principal alpha and modulatory beta subunits. The alpha subunits consist of CNG1-3 and form functional cation channels by themselves. The beta subunits consist of CNG4-6 and, unlike the alpha subunits, do not form functional channels, but rather modify the properties of channels. CNG channels are essential components of olfactory and visual transduction. In olfactory neurons, CNG2, CNG4.3 and CNG5 form Ca2+ permeable channels, which open and depolarize the cell in response to cAMP. In rod photoreceptors, CNG1 and CNG4.1 combine to form Ca ion permeable channels, which give rise to a current in response to cGMP. CNG3 and CNG6 are expressed in cone receptors and may combine to form a native cGMP-activated channel. CNG channels have been implicated in other areas. CNG1 is also expressed in medium-sized and small-sized arteries, suggesting a role for CNG in the regulation of arterial blood pressure and of blood supply to different regions. CNG1, CNG4.1 and CNG4.2 have been detected in the rat pineal gland. CNG2, CNG4.3 and CNG5 are present in GT1 cell lines and may play a role in the secretion of gonadotropin-releasing hormone.

產(chǎn)品規(guī)格：0.1ml/0.2ml

注意事項:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

此抗體本公司有單抗和多抗，可用于免疫組化（IHC），免疫印跡（WB），酶聯(lián)免疫(ELISA)，IF、IP、流式細胞術(shù)等實驗（可來電索取說明）
此產(chǎn)品本公司有標記的一抗出售，相關(guān)標記有：Alexa Fluor 350 標記、Alexa Fluor 488 標記、Alexa Fluor 555 標記、Alexa Fluor 647 標記、AP標記、APC標記、Biotin標記、Cy3標記、Cy5標記、Cy5.5標記、Cy7標記、FITC標記、Gold標記、HRP標記、PE標記、PE-Cy3標記、PE-CY5標記、PE-CY5.5標記、PE-CY7標記、RBITC標記

保存條件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Anti-CNG3抗體,視錐感光細胞環(huán)磷酸鳥苷門控通道蛋白CNG3抗體技術(shù)說明免疫組化染色：
SP法
（1） 脫蠟、水化 （2）PBS洗2-3次各5分鐘 （3）3%H2O2（80%甲醇）滴加在
TMA上，室溫靜置10分鐘 （4）PBS洗2-3次各5分鐘 （5）抗原修復(fù)
（6）PBS洗2-3次各5分鐘 （7）滴加正常山羊血清封閉液，室溫20分鐘，甩去多余液體（8）滴加Ι抗50μl,室溫靜置1小時或4℃過夜或37℃1小時（9）4℃過夜后需在37℃復(fù)溫45分鐘（10）PBS洗3次每次2分鐘
（11）滴加Ⅱ抗45-50μl,室溫靜置或37℃1小時（12）Ⅱ抗中可加入0.05%的
tween-20.（13）PBS洗3次各5分鐘（14）DAB顯色5-10分鐘，在顯微鏡下掌握染色程度 （15）PBS或自來水沖洗10分鐘 （16）蘇木精復(fù)染2分鐘，鹽酸酒精分化（17）自來水沖洗10-15分鐘 （18）脫水、透明、封片、鏡檢。
SABC法
（1） 脫蠟、水化 （2）PBS洗2次各5分鐘 （3）用蒸餾水或PBS配制新鮮的3%H2O2，室溫封閉5-10分鐘，用蒸餾水洗3次（4）抗原修復(fù)
（5） PBS洗5分鐘 （6）滴加正常山羊血清封閉液，室溫20分鐘，甩去多余液體（7）滴加Ι抗50μl,室溫靜置1小時或4℃過夜或37℃1小時
（8）PBS洗3次各2分鐘（9）滴加*化Ⅱ抗，20℃-37℃ 20分鐘
（10）PBS洗3次各2分鐘 （11）滴加試劑SABC 20℃-30℃ 20分鐘
（12） PBS洗4次各5分鐘 （13） DAB顯色：試劑盒或自配顯色劑顯色
（14）脫水、透明、封片、鏡檢。

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