產品名稱：Anti-LIM Kinase 1抗體,LIM結構域激酶1抗體

產品編號：BYK-18254R
英文名稱：Anti-LIM Kinase 1
中文名稱：LIM結構域激酶1抗體
英文別名：EC 2.7.1.37;

LIM domain containing protein kinase;

LIM domain kinase 1;

LIM motif containing protein kinase;

LIMK 1;

LIMK;

LIMK-1;

limk1;

LIMK1_HUMAN.
英文介紹：There are approximay 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

產品規格：0.1ml/0.2ml

注意事項:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

此抗體本公司有單抗和多抗，可用于免疫組化（IHC），免疫印跡（WB），酶聯免疫(ELISA)，IF、IP、流式細胞術等實驗（可來電索取說明）
此產品本公司有標記的一抗出售，相關標記有：Alexa Fluor 350 標記、Alexa Fluor 488 標記、Alexa Fluor 555 標記、Alexa Fluor 647 標記、AP標記、APC標記、Biotin標記、Cy3標記、Cy5標記、Cy5.5標記、Cy7標記、FITC標記、Gold標記、HRP標記、PE標記、PE-Cy3標記、PE-CY5標記、PE-CY5.5標記、PE-CY7標記、RBITC標記

保存條件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Anti-LIM Kinase 1抗體,LIM結構域激酶1抗體免疫組化操作流程
1 脫蠟和水化：脫蠟前應將組織芯片在室溫中放置60分鐘或60℃恒溫箱中烘烤20分鐘。 a 組織芯片置于二甲苯中浸泡10分鐘,更換二甲苯后在浸泡10分鐘
b 無水乙醇中浸泡五分鐘
c 95%乙醇中浸泡五分鐘
d 75%乙醇中浸泡五分鐘
2 抗原修復：用于福爾馬林固定的石蠟包埋組織芯片：
A 抗原熱修復
a 高壓熱修復 在沸水中加入EDTA（ph8.0）或0.01m*緩沖溶液（ph6.0）.蓋上不銹鋼鍋蓋，但不能鎖定。將玻片置于金屬染色加上，緩慢加壓，是玻片在緩沖液中浸泡五分鐘，然后將蓋子鎖定，小閥門將會升起來。10分鐘后除去熱源，置入涼水中，當小閥門沉下去后打開蓋子。此方法適用于較難檢測或核抗原的抗原修復。
b 沸熱修復 電爐或水浴鍋加熱0.01*緩沖液（ph6.0）至95℃左右，放入組織芯片加熱10-15分鐘。
C 微波爐加熱 在微波爐里加熱0.01*緩沖液（ph6.0）至沸騰后將組織芯片放入，斷電，間隔5-10分鐘，反復1-2次。適用的抗原：
Bcl-2.Bax.AR.PR.C-fos.x-jum.z-kit.c-myc.E-cadherin.
ChromograninA.Cyclin.ER.Heatshock.Protein.HPV.Ki-67.MDMZ.P53.P34.P15.P-glycoprotein.PKC.PCNA.ras.Rb.等
B 酶消化方法 
常用0.1%*和0.4%*液。*使用前預熱37℃，消化時間約為5-30分鐘。適用于被固定遮蔽的抗原：包括 Collagen.GFAP.
Complement.Cytokeratin.C-erB-2.LCA.LN.等

Anti-LIM Kinase 1抗體,LIM結構域激酶1抗體其他優勢產品：

byk-0611R 脂聯素受體2抗體 Anti-Adiponectin receptor 2
byk-0610R 脂聯素受體1抗體 Anti-Adiponectin Receptor 1
byk-11930R 胰腺特異轉錄因子PTF1A抗體 Anti-PTF1A
byk-1930R 抗利尿激素/血管升壓素/加壓素/血管加壓素抗體 Anti-ADH/AVP/ARVP
byk-1385R 整合素樣金屬蛋白酶與*1型-12抗體 Anti-ADAMTS12
byk-11385R 芳香乙酰胺脫乙酰基酶樣蛋白2抗體 Anti-AADACL2
byk-11673R *消旋酶 Anti-Serine racemase
byk-11164R 細胞粘附分子伴侶蛋白1抗體 Anti-Sidekick 1
byk-1164R 脂肪組織分化相關蛋白抗體 Anti-ADFP
byk-11114R 原鈣粘附蛋白8抗體 Anti-PCDH8
byk-1114R 內收蛋白抗體 Anti-beta Adducin
byk-12168R G蛋白偶聯受體18抗體 Anti-GPR18
byk-2168R 雙鏈RNA腺苷酸脫氨基酶抗體（C端） Anti-ADAR1 (C-terminus)
byk-12167R 磷酸化鉀通道相互作用蛋白3抗體 Anti-phospho-CSEN (Ser63)
byk-2167R 雙鏈RNA腺苷酸脫氨基酶抗體（N端） Anti-ADAR1 (N-terminus)
byk-0733R 整合素樣金屬蛋白酶與*1型-7抗體 (N端抗體) Anti-ADAMTS7(NT)
byk-0433R 整合素樣金屬蛋白酶與*1型-7抗體 Anti-ADAMTS7
byk-11208R 核轉錄因子SOX4抗體 Anti-SOX4
byk-1208R 整合素樣金屬蛋白酶與*1型抗體 Anti-ADAMTS1
byk-0720M 神經絲蛋白抗體 Anti-AD7C-NTP
byk-12105R TEX261蛋白抗體 Anti-TEX261
byk-10046R 肌營養蛋白α抗體 Anti-DRP3/Dystrobrevin alpha
byk-11571R 富含*重復跨膜神經元蛋白3抗體 Anti-LRRTM3
byk-1571R 纖維狀肌動蛋白抗體 Anti-F-Actin
byk-11741R ELAVL2蛋白抗體 Anti-ELAVL2/HUB
byk-1741R α-輔肌動蛋白4(內參)抗體 Anti-alpha Actinin 4-Loading Control
byk-0189R 肌動蛋白α/α-SMA/α Actin抗體 Anti-Actin

咨詢及訂購的方法：
1.可來電向我們直接咨詢及訂購。：021-6034 0820,158 0061 4926
2.可加客服或交談，向我司在線人員咨詢及訂購。
3.可通過郵件咨詢及訂購。