產品名稱：Anti-IL12 p40抗體,白細胞介素12p40抗體

產品編號：BYK-19133R
英文名稱：Anti-IL12 p40
中文名稱：白細胞介素12p40抗體
英文別名：CLMF;

CLMF p40;

CLMF2;

Cytotoxic lymphocyte maturation factor 40 kDa subunit;

IL 12 subunit p40;

IL 12B;

IL-12 subunit p40;

IL-12B;

IL12 subunit p40;

IL12B;

IL12B_HUMAN;

interleukin 12 beta chain;

Interleukin 12 p40;

Interleukin 12 subunit beta;

Interleukin 12B;

Interleukin-12 subunit beta;

natural killer cell stimulatory factor 40 kD subunit;

NK cell stimulatory factor chain 2;

NKSF;

NKSF2;

p40.
英文介紹：This gene encodes a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children.

產品規格：0.1ml/0.2ml

注意事項:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

此抗體本公司有單抗和多抗，可用于免疫組化（IHC），免疫印跡（WB），酶聯免疫(ELISA)，IF、IP、流式細胞術等實驗（可來電索取說明）
此產品本公司有標記的一抗出售，相關標記有：Alexa Fluor 350 標記、Alexa Fluor 488 標記、Alexa Fluor 555 標記、Alexa Fluor 647 標記、AP標記、APC標記、Biotin標記、Cy3標記、Cy5標記、Cy5.5標記、Cy7標記、FITC標記、Gold標記、HRP標記、PE標記、PE-Cy3標記、PE-CY5標記、PE-CY5.5標記、PE-CY7標記、RBITC標記

保存條件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Anti-IL12 p40抗體,白細胞介素12p40抗體免疫組化操作流程
1 脫蠟和水化：脫蠟前應將組織芯片在室溫中放置60分鐘或60℃恒溫箱中烘烤20分鐘。 a 組織芯片置于二甲苯中浸泡10分鐘,更換二甲苯后在浸泡10分鐘
b 無水乙醇中浸泡五分鐘
c 95%乙醇中浸泡五分鐘
d 75%乙醇中浸泡五分鐘
2 抗原修復：用于福爾馬林固定的石蠟包埋組織芯片：
A 抗原熱修復
a 高壓熱修復 在沸水中加入EDTA（ph8.0）或0.01m*緩沖溶液（ph6.0）.蓋上不銹鋼鍋蓋，但不能鎖定。將玻片置于金屬染色加上，緩慢加壓，是玻片在緩沖液中浸泡五分鐘，然后將蓋子鎖定，小閥門將會升起來。10分鐘后除去熱源，置入涼水中，當小閥門沉下去后打開蓋子。此方法適用于較難檢測或核抗原的抗原修復。
b 沸熱修復 電爐或水浴鍋加熱0.01*緩沖液（ph6.0）至95℃左右，放入組織芯片加熱10-15分鐘。
C 微波爐加熱 在微波爐里加熱0.01*緩沖液（ph6.0）至沸騰后將組織芯片放入，斷電，間隔5-10分鐘，反復1-2次。適用的抗原：
Bcl-2.Bax.AR.PR.C-fos.x-jum.z-kit.c-myc.E-cadherin.
ChromograninA.Cyclin.ER.Heatshock.Protein.HPV.Ki-67.MDMZ.P53.P34.P15.P-glycoprotein.PKC.PCNA.ras.Rb.等
B 酶消化方法 
常用0.1%*和0.4%*液。*使用前預熱37℃，消化時間約為5-30分鐘。適用于被固定遮蔽的抗原：包括 Collagen.GFAP.
Complement.Cytokeratin.C-erB-2.LCA.LN.等

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